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This detailed book presents a technical overview and practical methodology of a variety of antibody array formats and technologies. As advantages and disadvantages of antibody array types are explored, the volume also delves into practical applications of antibody arrays pertaining to investigations of specific research topics and biological processes as well as guidance on the methods of processing, analysis, and storage of array data. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Antibody Arrays: Methods and Protocols aims to empower the reader with the information required to select the most appropriate array for their research application, with the technical knowledge touse and process the array, and with the knowledge to perform analysis that realizes the maximum benefit from the data generated.
List of contents
Sandwich-Based Antibody Arrays for Protein Detection.- Well-Based Antibody Arrays.- One-Step Antibody Arrays.- Global Detection of Proteins by Label-Based Antibody Array.- Surface Plasmon Resonance Imaging-Mass Spectrometry Coupling on Antibody Array Biochip: Multiplex Monitoring of Biomolecular Interactions and On-Chip Identification of Captured Antigen.- Multiplexed Protein Biomarker Detection with Microfluidic Electrochemical Immunoarrays.- Cytometry Multiplex Bead Antibody Array.- Antibody Arrays: Barcode Technology.- Reverse Phase Protein Arrays.- Enhanced Protein Profiling Arrays for High-Throughput Quantitative Measurement of Cytokine Expression.- Methods for Membranes and Other Absorbent Surfaces.- Precise Chip-to-Chip Reagent Transfer for Cross-Reactivity-Free Multiplex Sandwich Immunoassays.- Antibody Printing Technologies.- Self-Assembling Peptide Hydrogels for 3D Microarrays.- Surface Modification of Glass Slides with Aminosilanes for Microarray Use.- Dried Blood-Based Protein Profiling Using Antibody Arrays.- Profiling Glycoproteins on Functionalized Reverse Phase Protein Array.- Determining Protein Phosphorylation Status Using Antibody Arrays and Phos-Tag Biotin.- Analyzing Signaling Pathways Using Antibody Arrays.- Using Antibody Arrays for Biomarker Discovery.- Immune Cell Isolation from Murine Intestine for Antibody Array Analysis.- Database Development for Antibody Arrays.- Data Analysis for Antibody Arrays.- Statistical Analysis Options for Antibody Array Data.
