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This volume explores various experimental approaches that can be used to investigate myeloid-derived suppressor cells (MDSC) and can be useful to understand the characterization of these cells. The chapters in this book cover topics such as the study of MDSC in human patient samples; employing flow cytometry or magnetic enrichment; immunophenotyping using multi-parameter flow cytometry and mass cytometry; functional characterization of MDSC by biochemical, immunological, and microscopy approaches; MDSC characterization through the use of high-resolution genomics; and novel methods of high-resolution visualization of living tissue and the purification of granulocytes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and thorough, Myeloid-Derived Suppressor Cells: Methods and Protocols is a valuable resource for novices in this field to help them start their research on MDSC, and experienced investigators looking to establish new protocols in their lab. This book is great for anyone working in the field of biology, medicine, and veterinary medicine.
List of contents
Immunophenotyping of Circulating Myeloid-Derived Suppressor Cells (MDSC) in the Peripheral Blood of Cancer Patients.- Phenotyping of Myeloid-Derived Suppressor Cells in Nonhuman Primates.- Isolation and Phenotyping of Splenic Myeloid-Derived Suppressor Cells in Murine Cancer Models.- Phenotypical Characterization and Isolation of Tumor-Derived Mouse Myeloid-Derived Suppressor Cells.- Isolation of Human Circulating Myeloid-Derived Suppressor Cells and Analysis of Their Immunosuppressive Activity.- High Dimensional Phenotyping of Human Myeloid-Derived Suppressor Cells/Tumor-Associated Macrophages in Tissue by Mass Cytometry.- Depletion and Maturation of Myeloid-Derived Suppressor Cells in Murine Cancer Models.- In Vitro Generation of Human Neutrophilic Myeloid-Derived Suppressor Cells.- Measuring Suppressive Activity and Autophagy in Myeloid-Derived Suppressor Cells.- In Vitro Generation of Murine Myeloid-Derived Suppressor Cells, Analysis of Markers, Developmental Commitment, and Function.- Analysis of Antimicrobial Activity of Monocytic Myeloid-Derived Suppressor Cells in Infection with Mycobacterium tuberculosis and Human Immunodeficiency Virus.- Isolation and Functional Characterization of Myeloid-Derived Suppressor Cells in Infections Under High Containment.- High-Dimensional Analysis of Circulating and Tissue-Derived Myeloid-Derived Suppressor Cells from Patients with Glioblastoma.- Single Cell Transcriptome Analysis Workflow for Splenic Myeloid-Derived Suppressor Cells from Murine Breast Cancer Models.- Intravital 2-Photon Microscopy of Diverse Cell Types in the Murine Tibia.- Isolation of Bovine Neutrophils by Fluorescence- and Magnetic-Activated Cell Sorting.
