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This volume describes a range of standard and novel methodological approaches used to probe ion channel function across different modalities. Chapters guide readers through methods and protocols from an introduction to the decades old patch clamp method for the ion channel neophyte to more complex, recent protocol advances, such as optogenetics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Patch Clamp Electrophysiology: Methods and Protocols aims to be a reference guide for current and future ion channel physiologists.
List of contents
An Introduction to Patch Clamp Recording.- Patch Clamp Technology in the 21st Century.- Heterologous Expression of Ion Channels in Mammalian Cell Lines.- Electrophysiology on Channel Forming Proteins In Artificial Lipid Bilayers: Next Generation Instrumentation For Multiple Recordings In Parallel.- Perforated Whole-Cell Recordings in Automated Patch Clamp Electrophysiology.- Multi-electrode Arrays.- Utilising Automated Electrophysiological Platforms in Epilepsy Research.- Dynamic Clamp on a Windows PC.- Combining Whole-cell Patch-clamp Techniques with Single-cell RNA Sequencing.- Bioluminescence Methodology for Ion Channel Studies.- Nucleated, Outside-Out, Somatic, Macropatch Recordings in Native Neurons.- Preparation of Rat Organotypic Hippocampal Slice Cultures Using the Membrane-Interface Method.- In Vivo Patch Clamp Studies.- In Vivo Optogenetics with Stimulus Calibration.- Single or Double Patch ClampRecordings in the Ex Vivo Slice Preparation: Functional Connectivity, Synapse Dynamics, and Optogenetics.- Optogenetics and Optical Tools in Automated Patch Clamping.
