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This volume details a comprehensive range of methods for imaging epithelial-to-mesechymal transition (EMT)/MET in in vivo systems, and methods to leverage these systems to dissect the underlying mechanisms. Chapters guide readers through studying different features of epithelial-mesenchymal plasticity, past and future research of the EMT, in vivo systems, and in vivo imaging. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, The Epithelial-to Mesenchymal Transition: Methods and Protocols aims to provide methods in EMT will help to unite and drive research in this exciting field forwards.
List of contents
A Spectrum of Cell States during the Epithelial-To-Mesenchymal Transition.- Perspective on Epithelial Mesenchymal Transitions in Embryos.- EMT, One of Many Morphological Transitions in Cellular Phase Space.- Are you Interested or Afraid of Working on EMT?.- Partial EMT/MET: An Army of One.- EMT; An Update.- Mesenchymal to Epithelial Transitions in Development and Cancer.- Live Imaging of Epithelial-mesenchymal Transition in Mesoderm Cells of Gastrulating Drosophila Embryos.- Zebrafish Neural Crest: Lessons and Tools to Study in vivo Cell Migration.- Live Imaging of the Neural Crest Cell Epithelial-to-Mesenchymal Transition in the Chick Embryo.- Exploiting Drosophila melanogaster Wing Imaginal Disc Eversion to Screen for New EMT Effectors.- Visualizing Mouse Embryo Gastrulation Epithelial-mesenchymal Transition through Single Cell Labeling Followed by ex vivo Whole Embryo Live Imaging.- Fluorescence Recovery after Photobleaching to Study the Dynamics of Membrane Bound Proteins in vivo using the Drosophila Embryo.- Methods to Generate and Assay for Distinct Stages of Cancer Metastasis in adult Drosophila melanogaster.- 4D Live-Imaging and Analysis of Chick Embryo Somites.- In vivo Analysis of the Mesenchymal-to-Epithelial Transition during Chick Secondary Neurulation.- Multiscale in vivo Imaging of Collective Cell Migration in Drosophila Embryos.- Methods to Generate Tube Micropatterns for Epithelial Morphogenetic Analyses and Tissue Engineering.- CAFs and Cancer Cells Co-Migration in 3D Spheroid Invasion Assay.- Using Xenopus Neural Crest Explants to Study Epithelial-mesenchymal Transition.- Xenopus Deep Cell Aggregates: a 3D Tissue Model for Mesenchymal-To-Epithelial Transition.- Molecular Tension Microscopy of E-cadherin during Epithelial-Mesenchymal Transition.- Methodologies for Following EMT in vivo at Single Cell Resolution.- Isolation and Identification of EMT Subtypes.- Studying the Metabolism of Epithelial-Mesenchymal Plasticity using the Seahorse XFe96 Extracellular Flux Analyzer.- Inducing Sequential Cycles of Epithelial-Mesenchymal and Mesenchymal-Epithelial Transitions in Mammary Epithelial Cells.- Analysis of cellular EMT States using Molecular Biology and High Resolution FISH Labeling.- Mathematical Modeling of Plasticity and Heterogeneity in EMT.- Control of Cell Migration Using Optogenetics.
