Diatom Microscopy

DIATOM MICROSCOPY
 
The main goal of the book is to demonstrate the wide variety of microscopy methods being used to investigate natural and altered diatom structures.
 
This book on Diatom Microscopy gives an introduction to the wide panoply of microscopy methods being used to investigate diatom structure and biology, marking considerable advances in recent technology including optical, fluorescence, confocal and electron microscopy, surface-enhanced Raman spectroscopy (SERS), atomic force microscopy (AFM) and spectroscopy as applied to diatoms. Each chapter includes a tutorial on a microscopy technique and reviews its applications in diatom nanotechnology and diatom research. The number of diatomists, diatom research, and their publications are increasing rapidly. Although many books have dealt with various aspects of diatom biotechnology, nanotechnology, and morphology, to our knowledge, no volume exists that summarizes advanced microscopic approaches to diatoms.
 
Audience
 

The intended audience is academic and industry researchers as well as graduate students working on diatoms and diatom nanotechnology, including biosensors, biomedical engineering, solar panels, batteries, drug delivery, insect control, and biofuels.

List of contents

Preface xi
 
1 Investigation of Diatoms with Optical Microscopy 1
Shih-Ting Lin, Ming-Xin Lee and Guan-Yu Zhuo
 
1.1 Introduction 2
 
1.2 Light Microscopy 4
 
1.2.1 Phase Contrast Microscopy 4
 
1.2.2 Differential Interference Contrast (DIC) Microscopy 6
 
1.2.3 Darkfield Microscopy 9
 
1.3 Fluorescence Microscopy 10
 
1.4 Confocal Laser Scanning Microscopy 12
 
1.5 Multiphoton Microscopy 19
 
1.6 Super-Resolution Optical Microscopy 22
 
1.7 Conclusion 25
 
Acknowledgement 25
 
References 25
 
2 Nanobioscience Studies of Living Diatoms Using Unique Optical Microscopy Systems 33
Kazuo Umemura
 
Abbreviations 33
 
2.1 Trajectory Analysis of Gliding Among Individual Diatom Cells Using Microchamber Systems 35
 
2.2 Direct Observation of Floating Phenomena of Individual Diatoms Using a "Tumbled" Microscope System 40
 
2.3 Three-Dimensional Physical Imaging of Living Diatom Cells Using a Holographic Microscope System 44
 
Acknowledgements 47
 
References 47
 
3 Recent Insights Into the Ultrastructure of Diatoms Using Scanning and Transmission Electron-Microscopy 57
Dharshini Gopal, Shweta Chakrabarti, Dataram Venkata Srikar Keshav, Richard Gordon and Nirmal Mazumder
 
3.1 Introduction 58
 
3.2 Scanning Electron Microscopy (SEM) of Diatoms 58
 
3.3 Transmission Electron Microscopy (TEM) of Diatoms 65
 
3.3.1 Limitations 69
 
3.4 Conclusion 73
 
References 73
 
4 Atomic Force Microscopy Study of Diatoms 81
Ishita Chakraborty, Shweta Chakrabarti, Vishwanath Managuli and Nirmal Mazumder
 
4.1 Introduction 82
 
4.2 Types of AFM Modes 86
 
4.3 Sample Preparation and Methods 88
 
4.4 Study of Diatom Ultrastructure Under AFM 88
 
4.5 Conclusion 102
 
Glossary 104
 
Acknowledgement 104
 
References 105
 
5 Refractive Index Tomography for Diatom Analysis 111
Juan M. Soto, José A. Rodrigo and Tatiana Alieva
 
5.1 Introduction 112
 
5.2 Fundamentals of PC-ODT 113
 
5.3 Experimental Setup for PC-ODT 117
 
5.4 Diatom RI Reconstructions with Bright-Field Illumination 120
 
5.5 Illumination Impact on PC-ODT Performance 126
 
5.6 Concluding Remarks 131
 
Acknowledgement 134
 
References 134
 
6 Luminescent Diatom Frustules: A Review on the Key Research Applications 139
Jayur Tisso, Shruthi Shetty, Nirmal Mazumder, Ankur Gogoi and Gazi A. Ahmed
 
6.1 Introduction 140
 
6.2 Key Research Applications of Luminescence Properties of Diatom Frustules 141
 
6.2.1 Novel Nanophotonic and Optoelectronic Applications of Luminescent Diatom Frustules 142
 
6.2.2 Applications of Diatom Luminescence in Sensing 145
 
6.2.3 Biomedical Applications of Diatom Luminescence 149
 
6.2.4 Other Studies on Diatom Luminescence 151
 
6.3 Future Perspectives 167
 
6.4 Conclusion 168
 
Acknowledgement 168
 
References 168
 
7 Micro to Nano Ornateness of Diatoms from Geographically Distant Origins of the Globe 179
Mohd Jahir Khan, Daniel Mathys and Vandana Vinayak
 
7.1 Introduction 180
 
7.2 Materials and Methods 183
 
7.2.1 Diatom Samples and Microscopy 183
 
7.2.1.1 By Michael J. Stringer 183
 
7.2.1.2 Diatom Oamaru Slides by Diane Winter 184
 
7.2.1.3 By Daniel Mathys 184
 
7.2.1.4 Diatom Sampling, Slide Preparation and Imaging from Himalayas, Plains and Arabian Sea, India 185
 
7.3 Diatoms from Different Geographical Origins of the World 185
 

About the author

Nirmal Mazumder obtained his PhD degree in 2013 from National Yang-Ming University, Taiwan. He has two years of post-doctoral research experience. In 2016, he joined the Department of Biophysics, Manipal School of Life Sciences, Manipal Academy of Higher Education, Manipal, India as an assistant professor. His research interests relate to the development of Stokes-Mueller-based light microscopy for tissue characterization and deep learning. He also investigates the photonics properties of diatoms as well as frustules for bio-photonics applications. He has published several peer-reviewed journal articles and book chapters.
Richard Gordon's involvement with diatoms goes back to 1970 with his capillarity model for their gliding motility, published in the Proceedings of the National Academy of Sciences of the United States of America. He later worked on a diffusion-limited aggregation model for diatom morphogenesis, which led to the first paper ever published on diatom nanotechnology in 1988. He organized the first workshop on diatom nanotech in 2003.

Summary

DIATOM MICROSCOPY

The main goal of the book is to demonstrate the wide variety of microscopy methods being used to investigate natural and altered diatom structures.

This book on Diatom Microscopy gives an introduction to the wide panoply of microscopy methods being used to investigate diatom structure and biology, marking considerable advances in recent technology including optical, fluorescence, confocal and electron microscopy, surface-enhanced Raman spectroscopy (SERS), atomic force microscopy (AFM) and spectroscopy as applied to diatoms. Each chapter includes a tutorial on a microscopy technique and reviews its applications in diatom nanotechnology and diatom research. The number of diatomists, diatom research, and their publications are increasing rapidly. Although many books have dealt with various aspects of diatom biotechnology, nanotechnology, and morphology, to our knowledge, no volume exists that summarizes advanced microscopic approaches to diatoms.

Audience

The intended audience is academic and industry researchers as well as graduate students working on diatoms and diatom nanotechnology, including biosensors, biomedical engineering, solar panels, batteries, drug delivery, insect control, and biofuels.

Product details

• Authors: Richard Gordon, Nirmal Mazumder, Nirmal Gordon Mazumder
• Assisted by: Gordon (Editor), Richard Gordon (Editor), Nirmal Mazumder (Editor)
• Publisher: Wiley, John and Sons Ltd
• Languages: English
• Product format: Hardback
• Released: 31.05.2022
• EAN: 9781119711537
• ISBN: 978-1-119-71153-7
• No. of pages: 500
• Series: Diatoms: Biology and Applications
• Subjects: Natural sciences, medicine, IT, technology > Biology; Nanotechnologie, Mikroskopie, Life Sciences, Microscopy, Biowissenschaften, Kieselalgen, Nanotechnology, Allg. Nanotechnologie, Phycology, Algenkunde, Diatomeen