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This third edition expands upon the previous edition with current, detailed methods on different aspects, and landmarks advancing knowledge on Salmonella research. With new chapters on molecular assays for detection, identification and serotyping of Salmonella, quantitative proteomic identification of host factors involved in salmonella infection, determination of antimicrobial resistance in salmonella, site-directed mutagenesis, chromosomal gene analysis, various microscopy methods to analyze salmonella interaction with host cells, in vitro modeling of gallbladder-associated salmonella colonization, and analysis of salmonella phages and prophages. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Salmonella: Methods and Protocols, Third Edition aims to provide methods that will have an impacted Salmonella research
List of contents
Next day Salmonella spp. Detection Method Based on real-time PCR for Foods.- Isolation of Salmonella spp. from Animal Feed.- Investigating Outbreaks of Salmonella Typhimurium using Case-control Studies, with a Reference to the One Health Approach.- Detection of Salmonella by the 3M Molecular Detection Assays - MDS® Method.- CRISPR-Typing of Salmonella Isolates.- CRISPR Typing of Salmonella Isolates from Different Resources.- Immunomagnetic Separation of Salmonella with Tailored Magnetic Micro and Nanocarriers.- Self-labeling Enzyme Tags for Translocation Analyses of Salmonella Effector Proteins.- Smartphone Based Paper Microfluidic Immunoassay of Salmonella and E. coli.- Correlative Light and Scanning Electron Microscopy to Study Interactions of Salmonella enterica with Polarized Epithelial Cell Monolayers.- Production of Murine Macrophagesfrom Hoxb8-immortalized Myeloblasts: Utility and use in the Context of Salmonella Infection.- In vitro Evaluation of Anti-biofilm Agents Against Salmonella enterica.- Generation of Random luxCDABE Transcripcional Fusions in the Genome of Salmonella enterica.- Vaccine Based On Outer Membrane Vesicles Using Hydrogels as Vaccine Delivery System.- Detection and Characterization of Salmonella enterica Serotypes by Simple PCR Technologies.- Determination of Antimicrobial Resistance of Salmonella in Pork.- Molecular typing of Salmonella by Pulsed-field Gel Electrophoresis.- Isolation and Identification of Salmonella in Pork.
