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This volume provides a varied selection of current methods and molecular probes used to generate, image and quantify ROS production and lipid peroxidation. Chapters guide readers through methods and protocols on in silico modelling of ROS dynamics in biological systems and a breaking proposal. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Reactive Oxygen Species: Methods and Protocols aims to ensure successful results in the further study of this vital field.
List of contents
Introduction to in silico Modeling to Study ROS Dynamics.- Assessment of ROS Production in Mitochondria of Live Cells.- Measurement of Superoxide Production in Acute Hypoxia by Fixed-Cell Microscopy.- A Photodynamic Tool to Promote a Sustained, ROS-Dependent Growth of Human Hair Follicles in ex vivo Culture.- Singlet Oxygen and Protochlorophyllide Detection in Arabidopsis thaliana.- Characterization of oxidative lipidomics and autophagy induction in Chlamydomonas reinhardtii under abiotic stress.- Fluorescence Detection of Increased Reactive Oxygen Species Levels in Saccharomyces cerevisiae at the Diauxic Shift.- Flow Cytometry and Confocal Microscopy for ROS Evaluation in Fish and Human Spermatozoa.- Measurement of Reactive Oxygen Species In Semen Samples Using Chemiluminescence.- Methods to Unravel Pathways of Reactive Oxygen Species in the Photodynamic Inactivation of Bacteria.- Revealing ROS Production by Antibiotics and Photosensitizers in Biofilms. A Fluorescence Microscopy Approach.- EPR Spectroscopy as a Method for ROS Quantification in Skin.- Detection of Superoxide Radical in Adherent Living Cells by Electron Paramagnetic Resonance (EPR) Spectroscopy Using Cyclic Nitrones.- Singlet Oxygen Quantum Yield Determination Using Chemical Acceptors.- A General Method to Establish the Relative Efficiency of Different Sonosensitizers to Generate ROS for SDT.- Lipid Peroxidation Assay using BODIPY-phenylbutadiene Probes:A Methodological Overview.- Use of Fluorescent Probes to Detect ROS in Photodynamic Therapy.
