Molecular Biotechnology - Principles and Applications of Recombinant DNA

Molecular Biotechnology
 
Molecular Biotechnology
 
Principles and Applications of Recombinant DNA
 
SIXTH EDITION
 
An authoritative introduction to the fast-changing world of molecular biotechnology
 
In continuous publication since 1994 and now in its sixth edition, Molecular Biotechnology: Principles and Applications of Recombinant DNA has been effective in introducing this complex field to students for more than 25 years. This textbook covers essentially every aspect of the field of molecular biotechnology, which is constantly changing and adapting in light of new advances.
 
This edition includes the latest techniques in DNA sequencing and genetic engineering of microbial, plant, and animal genomes, including human genome editing, as well as updates across many areas, such as:
* Immunological assays for disease diagnosis, more effective bacteriophage therapy, and new ways of dealing with antibiotic-resistant bacteria
* New and developing vaccines for influenza, tuberculosis, and emerging viral threats, including Zika and SARS-CoV-2
* Engineering bacteria to perform plastic degradation and green algae to produce hydrogen, altering amino acid biosynthesis, and creating designer cellulosomes
* Production of humanized monoclonal antibodies in plants, modifying hybrid plants to produce clonal hybrids, and protecting plants from viral and fungal diseases
 
Molecular Biotechnology features nearly 600 detailed figures and is an ideal textbook for undergraduate and graduate courses in introductory biotechnology, as well as courses dedicated to utilizing this technology, such as medical, agricultural, environmental, and industrial biotechnology applications.

List of contents

1.The Development of Molecular Biotechnology 1
 
Emergence of Molecular Biotechnology 1
 
Recombinant DNA Technology 3
 
Commercialization of Molecular Biotechnology 6
 
Concerns and Consequences 8
 
Summary 10
 
References 10
 
Review Questions 11
 
2.Fundamental Technologies 13
 
Molecular Cloning 13
 
Preparation of DNA for Cloning 13
 
Insertion of Target DNA into a Plasmid Vector 18
 
Transformation and Selection of Cloned DNA in a Bacterial Host 21
 
Cloning Eukaryotic Genes 26
 
Recombinational Cloning 30
 
Genomic Libraries 32
 
Genome Engineering Using CRISPR Technology 36
 
Polymerase Chain Reaction 39
 
Amplification of DNA by PCR 39
 
Cloning PCR Products 42
 
Quantitative PCR 44
 
Chemical Synthesis of Genes 46
 
Assembling Oligonucleotides into Genes 46
 
Assembling PCR Products into Genes 46
 
DNA Sequencing Technologies 48
 
Dideoxynucleotide Sequencing 50
 
Sequencing Using Reversible Chain Terminators 53
 
Single-Molecule Real-Time Sequencing 55
 
Nanopore Sequencing 56
 
Sequencing Whole Genomes 56
 
Preparation of Genomic DNA Sequencing Libraries 57
 
High-Throughput Next-Generation Sequencing 59
 
Genome Sequence Assembly 60
 
Sequencing Metagenomes 61
 
Genomics 62
 
Transcriptomics 65
 
Proteomics 70
 
Metabolomics 84
 
Summary 86
 
References 87
 
Review Questions 89
 
3.Production of Recombinant Proteins 91
 
Protein Production in Prokaryotic Hosts 91
 
Regulating Transcription 92
 
Increasing Translation Efficiency 96
 
Increasing Protein Stability 100
 
Increasing Protein Secretion 105
 
Facilitating Protein Purification 111
 
Integrating DNA into the Host Chromosome 114
 
Heterologous Protein Production in Eukaryotic Cells 119
 
Posttranslational Modification of Eukaryotic Proteins 120
 
General Features of Eukaryotic Expression Systems 123
 
Yeast Expression Systems 123
 
Baculovirus-Insect Cell Expression Systems 137
 
Mammalian Cell Expression Systems 145
 
Protein Engineering 156
 
Directed Mutagenesis 156
 
Random Mutagenesis 161
 
Examples of Protein Engineering 164
 
Summary 173
 
References 174
 
Review Questions 176
 
4.Molecular Diagnostics 179
 
Immunological Approaches To Detect Protein Biomarkers 180
 
Antibodies 180
 
Agglutination 185
 
Enzyme-Linked Immunosorbent Assays 186
 
Protein Arrays To Detect Polygenic Diseases 194
 
Immunoassays for Protein Conformation-Specific Disorders 197
 
DNA-Based Diagnostic Approaches 199
 
Hybridization Probes 199
 
PCR-Based Detection Methods 207
 
CRISPR-Cas-Based Diagnostic Assays 218
 
DNA Microarrays 219
 
Whole-Genome Sequencing To Assess Genetic Disease Risk 225
 
Detecting RNA Signatures of Disease 226
 
Detection of Disease-Associated Changes in Gene Expression 227
 
Detection of RNA Signatures of Antibiotic Resistance in Bacteria 228
 
Detection of miRNA Signatures of Disease 230
 
Biofluorescent and Bioluminescent Systems 233
 
Fluorescent Proteins 233
 
Luciferase 234
 
Microbial Biosensors 235
 
Summary 238
 
References 239
 
Review Questions 241
 
5.Protein Therapeutics 243
 
Pharmaceuticals 244

About the author

About the Authors
Bernard R. Glick, PhD, is a distinguished professor emeritus at the University of Waterloo, Ontario, Canada, where he was the chair of biology from 2002 to 2008. He has authored more than 400 publications that have garnered over 54,000 citations. Cheryl L. Patten, PhD, is a professor of microbiology in the Biology Department at the University of New Brunswick, Fredericton, Canada. She teaches courses in introductory biology, microbiology, and biotechnology.

Summary

Molecular Biotechnology

Molecular Biotechnology

Principles and Applications of Recombinant DNA

SIXTH EDITION

An authoritative introduction to the fast-changing world of molecular biotechnology

In continuous publication since 1994 and now in its sixth edition, Molecular Biotechnology: Principles and Applications of Recombinant DNA has been effective in introducing this complex field to students for more than 25 years. This textbook covers essentially every aspect of the field of molecular biotechnology, which is constantly changing and adapting in light of new advances.

This edition includes the latest techniques in DNA sequencing and genetic engineering of microbial, plant, and animal genomes, including human genome editing, as well as updates across many areas, such as:
* Immunological assays for disease diagnosis, more effective bacteriophage therapy, and new ways of dealing with antibiotic-resistant bacteria
* New and developing vaccines for influenza, tuberculosis, and emerging viral threats, including Zika and SARS-CoV-2
* Engineering bacteria to perform plastic degradation and green algae to produce hydrogen, altering amino acid biosynthesis, and creating designer cellulosomes
* Production of humanized monoclonal antibodies in plants, modifying hybrid plants to produce clonal hybrids, and protecting plants from viral and fungal diseases

Molecular Biotechnology features nearly 600 detailed figures and is an ideal textbook for undergraduate and graduate courses in introductory biotechnology, as well as courses dedicated to utilizing this technology, such as medical, agricultural, environmental, and industrial biotechnology applications.

Product details

• Authors: Bernard R Glick, Bernard R. Glick, Cheryl L Patten, Cheryl L. Patten
• Publisher: Wiley & Sons
• Languages: English
• Product format: Hardback
• Released: 16.05.2022
• EAN: 9781683673644
• ISBN: 978-1-68367-364-4
• No. of pages: 896
• Dimensions: 218 mm x 48 mm x 282 mm
• Weight: 2684 g
• Series: ASM Books; ASM
• Subjects: Natural sciences, medicine, IT, technology > Biology > Microbiology; Biotechnologie, Medizin, Molekularbiologie, Life Sciences, Biowissenschaften, molecular biology, biotechnology, Biomedical engineering, Medical Science, Biotechnologie i. d. Biowissenschaften, Biomedizintechnik, Molekulares Bioengineering, Molecular Bioengineering