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Dieser umfassende Band beschreibt viele der aktuell angewandten Techniken zur Bestimmung der molekularen Mechanismen, die bei der Rezeptorregulation eine Rolle spielen. Behandelt wird ein breites Spektrum wichtiger Grundlagen und Methoden für die Analyse von G Protein-gekoppelten Rezeptoren. Geschrieben von anerkannten Experten auf diesem Gebiet. (01/00)
List of contents
TECHNIQUES FOR CHARACTERIZING RECEPTOR FUNCTION.
Epitope Tagging and Detection Methods for Receptor Identification (U. Klein & M. von Zastrow).
Measurement of Receptor Phosphorylation in Situ (M. Bouvier, et al.).
Reconstitution and in vitro Phosphorylation of G Protein-Coupled Receptors (J. Ptasienski & M. Hosey).
Identification of Residues that are Phosphorylated within a Receptor (K. Palczewski, et al.).
Palmitoylation of G Protein-Coupled Receptors (H. Jin, et al.).
Assays for Measuring Receptor/G Protein Coupling (Q. Li & R. Cerione).
PROTEINS THAT REGULATE RECEPTOR FUNCTION.
Expression and Activity of G Protein-Coupled Receptor Kinases (R. Loudon, et al.).
Characterization of Arrestin Expression and Function (V. Gurevich, et al.).
The G Protein-Coupled Receptor Phosphatase (J. Pitcher & R. Lefkowitz).
REGULATION OF RECEPTOR TRAFFICKING AND EXPRESSION.
Radioligand Binding Assays for G Protein-Coupled Receptor Internalization (M. Toews).
Characterization of Receptor Sequestration by Immunofluorescent Microscopy (F. Santini & J. Keen).
Characterization of Receptor Trafficking Using Green Fluorescent Protein (N. Tarasova, et al.).
The Analysis of Post-Transciptional Regulation of the Expression of G Protein-Coupled Receptors (B. Tholanikunnel & C. Malbon).
Index.
About the author
Jeffrey L. Benovic is the author of Regulation of G Protein Coupled Receptor Function and Expression: Receptor Biochemistry and Methodology, published by Wiley.
Summary
G Protein Coupled Receptors (GPCR) often play key roles in a large number of physiological and pathophysiological conditions. Theses receptors are the targets of a large number of therapeutic drugs. Detailed knowledge about the molecular structure of GPCRs should pave the way for more efficient drugs.
