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The Chlamydomonas Sourcebook, 3rd Edition Introduction to Chlamydomonas and Its Laboratory Use (Volume 1) The gold-standard reference covering the basic biology of the Chlamydomonas alga and techniques for its laboratory analysis Originally published as the standalone Chlamydomonas Sourcebook, then expanded as the first volume in a three-part comprehensive gold-standard reference, The Chlamydomonas Sourcebook: Introduction to Chlamydomonas and Its Laboratory Use has been fully revised and updated to include a wealth of new resources for the Chlamydomonas community. Early chapters cover current understandings of its taxonomy, ultrastructure, cell and life cycles, and nuclear and organelle genomes, followed by technique-oriented chapters covering such topics as cell culture, mutagenesis, genetic analysis, construction of mutant libraries, and protein localization using immunofluorescence. This volume presents the latest in research and best practices, making it a must-have resource for researchers and students working in plant science and photosynthesis, fertility, mammalian vision, and biochemistry; crop scientists; plant physiologists; and plant, molecular, and human disease biologists.
List of contents
1. The Genus Chlamydomonas
2. Cell Ultrastructure
3. Cell Walls
4. Functional Genomics
5. The Genome
6. Gene Expression: From Transcription to Alternative Splicing
7. Organelle Heredity
8. Cell Cycle and Circadian Rhythms
9. The Sexual Cycle
10. Multicellular Relatives of Chlamydomonas
11. Growth Techniques
12. Practical Aspects of Mating and Tetrad Analysis
13. Transformation of Nuclear, Chloroplast, and Mitochondrial Genomes
14. Methods for the Localization of Cellular Components
15. Mutagenesis and Genome Resequencing
16. Generation, storage, and utilizations of mutant libraries
17. Reverse Genetics
18. Transgene Design
About the author
Ursula Goodenough?is Professor of Biology Emerita at Washington University in St. Louis. She earned her PhD at Harvard University, where her thesis and postgraduate work focused on the biogenesis and ultrastructure of the Chlamydomonas chloroplast. In 1971, she initiated a career-spanning analysis of the Chlamydomonas sexual cycle and cell wall using a combination of genetics, ultrastructure, and molecular biology. She also used these approaches to study Chlamydomonas cilia and lipid-body formation. Key collaborators included Steven Adair, Patrick Ferris, Sabine Waffenschmidt, James Umen, and Jae-Hyeok Lee. Since retiring in 2017, she has completed a wide-ranging ultrastructural study of lichens.
