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This volume described basic and advanced protocols to study F. hepatica parasite biology. Chapters guide readers through protocols on different developmental stages of F. hepatica, characterize tegumental, secreted proteins, spatial and temporal gene expression, immunological effects of ESP on macrophages, eosinophil apoptosis, macrophages alternative activation, toll-like receptor interactions, obtain peritoneal and splenic dendritic cells of mice, and protocols to detect F. hepatica albendazole resistance. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Fasciola hepatica: Methods and Protocols provides different topics in areas such a biochemistry,immunology, molecular biology, microscopy, vaccinology in order to extend the interest in this book to research community working with Fasciola hepatica and related trematodes.
List of contents
Maintenance of Life Cycle at the Laboratory.- Microscopical Techniques to Analyse the Hepatic and Peritoneal Changes Caused by Fasciola hepatica Infection.- Isolation of Secreted and Tegumental Surface Proteins from Fasciola hepatica.- Isolation and Analysis of Fasciola hepatica Extracellular Vesicles.- Cloning and Heterologous Expression of Protein-Coding Sequences in Escherichia coli.- Gene Silencing in the Liver Fluke Fasciola hepatica: RNA Interference.- Analysis of Gene Expression in Fasciola hepatica Juveniles and Adults by In Situ Hybridization.- Evasion of Host Immunity during Fasciola hepatica Infection.- Immunomodulatory Effect of Fasciola hepatica Excretory-secretory Products on Macrophages.- Study of Eosinophil Apoptosis Induced by Fasciola hepatica Excretory-secretory Products.- Purification of native Fasciola hepatica Fatty Acid Binding Protein and the Induction of Alternative Activation of Human Macrophages.- Possible Role for Toll-Like Receptors in Interaction of Fasciola hepatica Excretory-Secretory Products with Human Monocyte Cell Line.- Evaluation of the Immune Regulatory Properties of Dendritic Cells during Fasciola hepatica Infection.- Design of a Peptide-carrier Vaccine Based on the Highly Immunogenic Fasciola hepatica Leucine Aminopeptidase.- Liver Fluke Vaccine Assessment in Cattle.- Testing Albendazole Resistance in Fasciola hepatica.- Drug Targets: Screening for Small Molecules that Inhibit Fasciola hepatica Enzymes.
