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Rare-Earth Element Biochemistry: Characterization and Applications of Lanthanide-Binding Biomolecules, Volume 651 in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Chapters in this new release include Spectrophotometric methods to probe the solution chemistry of lanthanide complexes with macromolecules, Determination of affinities of lanthanide-binding proteins using chelator-buffered titrations, Electron Paramagnetic Resonance of Lanthanides, Characterization of lanthanoid binding proteins using NMR spectroscopy, Macromolecular crystallography for f-element complex characterization, Infrared spectroscopy probes ion binding geometries, Predicting lanthanide coordination structures in solution with molecular simulation, and much more.
Additional sections cover the Characteristics of Gd(III) spin labels for the study of protein conformations, Lanthanide-based resonance energy transfer biosensors for live-cell applications, Yttrium-86 PET imaging, Aqueous Chemistry of the Smallest Rare Earth: Comprehensive Characterization of Radioactive and Non-radioactive Scandium Complexes for Biological Applications, and In vitro selection and application of lanthanide-dependent DNAzymes.
List of contents
1. Spectrophotometric methods to probe the solution chemistry of lanthanide complexes with macromolecules
Gauthier Deblonde
2. Determination of affinities of lanthanide-binding proteins using chelator-buffered titrations
Joseph A. Cotruvo Jr.
3. Electron Paramagnetic Resonance of Lanthanides
Gareth R. Eaton
4. Characterization of lanthanoid binding proteins using NMR spectroscopy
Claudio Luchinat and Giacomo Parigi
5. Macromolecular crystallography for f-element complex characterization
Rebecca Abergel
6. Infrared spectroscopy probes ion binding geometries
Carlos R. Baiz
7. Predicting lanthanide coordination structures in solution with molecular simulation
David Cantu
8. Characteristics of Gd(III) spin labels for the study of protein conformations
Daniella Goldfarb
9. Lanthanide-based resonance energy transfer biosensors for live-cell applications
Lawrence W. Miller
10. Yttrium-86 PET imaging
Peter Caravan and Mariane Le Fur
11. Aqueous Chemistry of the Smallest Rare Earth: Comprehensive Characterization of Radioactive and Non-radioactive Scandium Complexes for Biological Applications
Eszter Boros
12. In vitro selection and application of lanthanide-dependent DNAzymes
Juewen Liu
About the author
Joseph A. Cotruvo, Jr. is Assistant Professor, Louis Martarano Career Development Professor, Department of Chemistry
Pennsylvania State University, PA, USA
