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Enzymatic Plastic Degradation, Volume 648 in the Methods in Enzymology series, continues the legacy of this premier serial with chapters authored by leaders in the field. Chapters in this latest release include Evaluating plastic pollution and environmental degradation, Assessment methods for microplastic pollution in the oceans and fresh water, Exploring microbial consortia from various environments for plastic degradation, Characterization of filamentous fungi for attack on synthetic polymers via biological Fenton chemistry, Synthesis of radioactive-labeled nanoplastics for assaying the environmental (microbial) PS degradation, Exploring metagenome for plastic degrading enzymes, Cutinases from thermophilic bacteria (actinomycetes): from identification to functional and structural characterization, and much more.
List of contents
1. Evaluating plastic pollution and environmental degradation
Pia Skoczinski
2. Assessment methods for microplastic pollution in the oceans and fresh water
Chengjun Sun
3. Exploring microbial consortia from various environments for plastic degradation
Basak Öztürk and Ingrid Eileen Meyer Cifuentes
4. Characterization of filamentous fungi for attack on synthetic polymers via biological Fenton chemistry
Dietmar Schlosser
5. Culturing various mealworms for the degradation of PS/PE and the corresponding analysis of gut microbiota
Wei-Min Wu
6. Synthesis of radioactive-labeled nanoplastics for assaying the environmental (microbial) PS degradation
Rong Ji
7. Exploring metagenome for plastic degrading enzymes
Wolfgang Streit
8. Cutinases from thermophilic bacteria (actinomycetes): from identification to functional & structural characterization
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9. Ideonella sakaiensis & PETase and MHETase: from identification of microbial PET degradation to enzyme characterization
Kohei Oda
10. Design of novel PETase mutants by machine-learning/AI-based strategy
Bian Wu
11. High throughput screening assays for PET/PU hydrolase activities
Ren Wei
12. Methods for evaluating the biodegradability of PET
Chen Song
13. Heterogeneous kinetic analysis of the enzymatic PET hydrolysis
Thomas Maskow
14. Adhesion peptide promoted and selective degradation of mixed plastics for enabling recycling
Shohana Subrin Islam
15. Adsorption study of plastic degrading enzymes and monomer conversion for synthesis of virgin polymers
Georg M. Guebitz
16. Characterization and screening of microbial degradation of PU
Luc Averous
17. Structural and functional characterization of the PET oligomer degrader (MHETase, Carboxylesterases, etc.)
Gert Weber and Gottfried J. Palm
18. Structural and functional characterization of nylon hydrolases
Seiji Negoro
19. Bioprocess engineering for PET recycling
Alain Marty
20. Engineering enzymes for embedding in PLA mulching films for rapid environmental degradation
Alain Marty
21. The use of degraded plastic monomers as substrates for microbial growth and production of biodegradable plastics (and their biodegradation)
Tanja Narancic
22. Culturing and engineering bacterial strains for up-cycling of plastic degradation products
Till Tiso
23. Screening and cultivating microbial strains able to grow on degradation intermediates from polyurethane
Christian Eberlein
24. Engineering microalgae as a whole cell biocatalyst for PET degradation
Daniel Moog
25. Yeast Cell Surface Display Bacterial PETase as a Reusable Biocatalyst for Degradation of Highly Crystallized Polyethylene Terephthalate
Zefang Wang
About the author
Gert Weber is at Helmholtz-Zentrum Berlin, GermanyProf. Uwe Bornscheuer from Greifswald University is a leading expert in enzymatic lipid modification as documented in numerous peer-reviewed publications, reviews as well as patents. He has received several scientific awards including the Chevreul Medal (French Lipid Society, 2012), the Normann Medal (DGF, Germany, 2014) and the Stephen S. Chang Award (AOCS, 2015).Ren Wei is at Universität Greifswald, Germany
