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This volume provides current management and research protocols on oesophageal squamous cell carcinoma. Chapters guide readers through methods on clinical and pathological diagnostics, translational research for oesophageal squamous cell carcinoma, in vitro assays, liquid biopsy for cancer DNA and circulating tumour cells, genomic analysis, mi-RNAs, and proteins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Esophageal Squamous Cell Carcinoma: Methods and Protocols to ensure successful results in the further study of this vital field.
List of contents
Introduction: Oesophageal Squamous Cell Carcinoma: Current Status and Future Advances.- Histopathological Assessment for Oesophageal Squamous Cell Carcinoma.- Application of Pathological Staging in Oesophageal Squamous Cell Carcinoma.- Macroscopic Examination of Surgical Specimen of Oesophageal Squamous Cell Carcinoma.- Endoscopic Diagnosis and Treatment of Oesophageal Squamous Cell Carcinoma.- Macroscopic Assessment and Sampling of Endoscopic Resection Specimens for Squamous Epithelial Malignancies with Superficial Involvement of Oesophagus.- Roles of Pathological Assessments of Frozen Sections in Oesophageal Squamous Cell Carcinoma.- Biobanking for Oesophageal Squamous Cell Carcinoma.- Whole-slide Imaging of Oesophageal Squamous Cell Carcinoma.- Use of Tissue Microarray in Oesophageal Squamous Cell Carcinoma.- Patient-derived Xenograft and Mice Models in Oesophageal Squamous Cell Carcinoma.- Orthotopic Xenograft Mouse Modelin Oesophageal Squamous Cell Carcinoma.- In Vitro Assays of Biological Aggressiveness of Oesophageal Squamous Cell Carcinoma.- Detention and Identification of Cancer Stem Cells in Oesophageal Squamous Cell Carcinoma.- Liquid Biopsy: Detection of Circulating Tumour Cells in Oesophageal Squamous Cell Carcinoma.- Liquid Biopsy for Investigation of Cancer DNA in Oesophageal Squamous Cell Carcinoma.- Genome Sequencing in Oesophageal Squamous Cell Carcinoma.- Roles of microRNAs in Oesophageal Squamous Cell Carcinoma Pathogenesis.- Mass-spectrometry for Biomarkers Discovery in Oesophageal Squamous Cell Carcinoma.- Immunoblotting in Detection of Tumour-associate Antigens in Oesophageal Squamous Cell Carcinoma.- Immunohistochemistry for Protein Detection in Oesophageal Squamous Cell Carcinoma.- Radiotherapy for Cervical Oesophageal Squamous Cell Carcinoma.- Radiotherapy for Thoracic Oesophageal Squamous Cell Carcinoma.- SystemicTherapy for Oesophageal Squamous Cell Carcinoma.- Surgical Protocols for Squamous Cell Cancer of the Oesophagus.- Anaesthetic Management for Squamous Cell Carcinoma of the Oesophagus.
