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This second edition volume delivers updated and new chapters on xenotransplantation covering a variety of methods. Despite many technological challenges faced by the xenotransplantation field, many major advances have been made in the last three decades. Expert researchers provide characterizations of xenogeneic interactions at the cellular and molecular levels, detailed descriptions of complex transplantation models, as well as attractive new tools for further advancement including 3D cell-culturing systems and genomic editing. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Thorough and intuitive, Xenotransplantation: Methods and Protocol, Second Edition aids scientists in continuing to study xenotransplantation and its multiple aspects.
List of contents
Introduction: The Present Status of Xenotransplantation Research.- Tools for Molecular Studies in Xenotransplantation.- Proteomic Protocols for Differential Protein Expression Analyses.- Specific Detection of Neu5Gc in Animal Tissues by Immunohistochemistry.- Xenoantibodies and Complement Activity Determinations by Flow Cytometry in Pig-to-Primate Xenotrasplantation.- 3D Cell-Culture Models for the Assessment of Anticoagulant and Anti-inflammatory Properties of Endothelial Cells.- Cell-based Assays for Modeling Xenogeneic Immune Responses.- Determination of Redox Status in Serum.- Generation of Genetically Modified Mice using CRISPR/Cas9.- Genome-wide PERV Inactivation in Pigs using CRISPR cas9.- Co-transplantation of Vascularized Thymic Graft with Kidney in Pig-to-Non-human Primates for the Induction of Tolerance Across Xenogeneic Barriers.- Xenogeneic Lung Transplantation Models.- Liver Xenotransplantation in a Non-humanPrimate Model.- Intra-bone Bone Marrow Transplantation in Pig-to-Non-human Primates for the Induction of Tolerance Across Xenogeneic Barriers.- Studying Xenograft Rejection of Bioprosthetic Heart Valves.- Corneal Xenotransplantation: Anterior Lamellar Keratoplasty.- Rat Model of Intra-articular Chondrocyte Xenotransplantation.- Porcine Hepatocytes: Isolation and Liver Tissue Engineering for Xenotransplantation.- Pig-to-Macaque Islet Xenotransplantation.- Regulation of Clinical Xenotransplantation: A Reappraisal of the Legal, Ethical and Social Aspects Involved.
