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This volume details state-of-the-art methods for the study of plant embryogenesis in the model organism Arabidopsis thaliana, other models, and non-model species. Chapters guide readers through genetic screens, phenotypic analysis, live imaging, transcriptional profiling, methods on other model and non-model species beyond Arabidopsis thaliana, and introduction to systems that allow to culture or produce zygotic and somatic embryos in vitro. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Plant Embryogenesis: Methods and Protocols aims to ensure successful results in the further study of this vital field.
The chapter "Small RNA In Situ Hybridizations on Sections of Arabidopsis Embryos" is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
List of contents
Genetic Screens to Target Embryo and Endosperm Pathways in Arabidopsis and Maize.- EMS Mutagenesis of Arabidopsis Seeds.- EMS Mutagenesis of Maize Pollen.- Live-cell Imaging of Zygotic Intracellular Structures and Early Embryo Pattern Formation in Arabidopsis thaliana.- Analyzing Sub-Cellular Reorganization during Early Arabidopsis Embryogenesis using Fluorescent Markers.- From Stained Plant Tissues to Quantitative Cell Segmentation Analysis with MorphoGraphX.- Small RNA In Situ Hybridizations on Sections of Arabidopsis Embryos.- Manual Isolation of Living Early Embryos from Tobacco Seeds.- Profiling Transcriptomes of Manually Dissected Arabidopsis Embryos.- Laser-assisted Microdissection of Plant Embryos for Transcriptional Profiling.- Cell-type-specific Transcriptomics in the Plant Embryo Using an Adapted INTACT Protocol.- Transcriptomic Profiling of the Arabidopsis Embryonic Epidermis UsingFANS in Combination with RNAseq.- Visualizing Protein Associations in living Arabidopsis Embryo.- Imaging of Embryo Sac and Early Seed Development in Maize after Feulgen Staining.- Using Giant Scarlet Runner Bean (Phaseolus coccineus) Embryos to Dissect the Early Events in Plant Embryogenesis.- Microscopical Detection of Cell Death Processes during Scots Pine Zygotic Embryogenesis.- Regulation of Somatic Embryo Development in Norway Spruce.- In vitro Production of Zygotes by Electro-Fusion of Rice Gametes.- Isolated Microspore Culture in Brassica napus.- Anther Culture in Eggplant (Solanum melongena L.).
