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This book provides a variety of procedures for synthetically producing peptides and their derivatives, ensuring the kind of precision that is of paramount importance for successful synthesis. Numerous techniques relevant to drugs and vaccines are explored, such as conjugation and condensation methodologies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Peptide Synthesis: Methods and Protocols serves as an essential guide to the many crucial processes that will allow researchers to efficiently prepare, purify, characterize, and use peptides for chemical, biochemical, and biological studies.
List of contents
Solution Phase Peptide Synthesis: The Case of Biphalin.- Application of Fmoc-SPPS, Thiol-Maleimide Conjugation and Copper(I)-Catalyzed Alkyne-Azide Cycloaddition "Click" Reaction in the Synthesis of a Complex Peptide-Based Vaccine Candidate against Group A Streptococcus.- In Situ Neutralization Protocols for Boc-SPPS.- Anhydrous Hydrogen Fluoride Cleavage in Boc Solid Phase Peptide Synthesis.- Automated Solid-Phase Peptide Synthesis.- New Developments in Microwave-Assisted Solid Phase Peptide Synthesis.- Protecting Groups in Peptide Synthesis.- Synthesis of O-Acyl Isopeptides: Stepwise and Convergent Solid-Phase Synthesis.- An Isodipeptide Building Block for Microwave-Assisted Solid-Phase Synthesis of Difficult Sequence-Containing Peptides.- SPOT Synthesis: The Solid-Phase Peptide Synthesis on Planar Surfaces.- Solid-Phase Synthesis of Wollamide Cyclohexapeptide Analogs.- Synthesis of Branched Peptides via a Side-Chain Benzyl Ester.- Solid Phase Synthesis of Octapeptin Lipopeptides.- Peptidomimetic Synthesis Drug Discovery for Alzheimer's Disease.- Synthesis of Amide Backbone-Modified Peptides.- Synthesis of NickFects, a New Family of CPPs, by Solid-Phase Peptide Synthesis.- Chemoenzymatic Synthesis of HIV-1 Glycopeptide Antigens.- Synthesis of Antimicrobial Lipopeptides Using the "CLipPA" Thiol-Ene Reaction.- Decarboxylative Couplings for Late-Stage Peptide Modifications.- Peptide and Pseudopeptide Bond Synthesis in Phosphorus Dipeptide Analogs.- Micro-Bioreactor Techniques for the Production and Spectroscopic Characterization of Microbial Peptides.- Metal Peptide Complexes for Study Neurodegenerative Diseases.
