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This volume provides a comprehensive overview of different ways to grow algae and the techniques used to start algal cultivation, monitor algal growth, environmental impact of growing algae, and various methods for characterizing the biomass. The chapters in this book discuss a range of topics such as step-by-step protocols on how to isolate and grow algae; descriptions on how to use fluorescence to estimate algal biomass and lipid content; and instructions on using advanced techniques to determine the carbohydrates and lipids in algal biomass. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Biofuels from Algae: Methods and Protocols is a valuable resource for novice and experienced researchers who want to learn more about this impactful and developing field.
List of contents
Large Scale Cultivation of Microalgae: Open and Closed Systems.- Design and Analysis of Offshore Macroalgae Biorefineries.- Basic Methods for Isolating and Culturing Microalgae.- Measurement of Fluorescence for Monitoring Algal Growth and Health.- Microalgae Lipid Staining with Fluorescent BODIPY Dye.- Monitoring Microalgal Neutral Lipid Accumulation with Nile Red.- Determining Inorganic and Organic Carbon.- Determining Inorganic and Organic Nitrogen.- Total Nitrogen Determination by a Spectrophotometric Method.- Determining Inorganic and Organic Phosphorus.- Determining Dissolved and Biogenic Silica.- Life Cycle Assessment of Seaweed Cultivation Systems.- Life Cycle Analysis in Algae Fuels.- Metabolic Engineering for Microalgae for Biofuels Production.- Analytical Grade Purification of Phycocyanin from Cyanobacteria.- Complete Acid Based Hydrolysis Assay for Carbohydrate Quantification in Seaweed: A Species Specific Optimized Approach.- Total Carbohydrate Content Determination of Microalgal Biomass by Acid Hydrolysis followed by Spectrophotometry or Liquid Chromatography.- Total Fatty Acid Content Determination of Whole Microalgal Biomass using In Situ Transesterification.- Liquid Chromatography-Mass Spectrometry (LC-MS)-Based Analysis of Molecular Lipids in Algae Samples.- UPLC-ELSD Analysis of Algal Lipid Classes and Derivatization of Bound and Free Fatty Acids and Sterols for GC-MS Methods.- Total Protein Content Determination of Microalgal Biomass by Elemental Nitrogen Analysis and a Dedicated Nitrogen-To-Protein Conversion Factor.
