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This volume looks at the latest technologies and methods used by researchers to study mechanisms that control the aspects of plant stem cell signaling events. The topics discussed in this book cover shoot apical meristem inoculation assay; plant associated microbes that alter root growth by modulating root apical meristem; visualizing auxin and cytokinins signaling activity; 3D analysis of mitosis distribution pattern in the plant root tip with iRoCS toolbox; and micropropagation of rosaceous species SAM grown in temperate climate. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and authoritative, Plant Stem Cells: Methods and Protocols is a useful resource for all researchers interested in learning more about this developing field.
List of contents
Regulatory Role of Phytohormones in Maintaining Stem Cells and Boundaries of Stem Cell Niches.- Bacterial Shoot Apical Meristem Inoculation Assay.- Germline-Transmitted Genome Editing Methodology in Arabidopsis thaliana using TAL-Effectors Nucleases.- Method to Study Gene Expression Patterns during de novo Root Regeneration from Arabidopsis Leaf Explants.- Labeling and Sorting of Arabidopsis SAM Cell Populations to Capture their Transcriptome Profile.- Plant Associated Microbes Alter Root Growth by Modulating Root Apical Meristem.- Live Imaging of Arabidopsis Axillary Meristems.- Molecular Modeling of the Interaction between Stem Cells Peptide and Immune Receptor in Arabidopsis.- Methods to Visualize Auxin and Cytokinins Signaling Activity in the Shoot Apical Meristem.- Analysis of Cell Division Frequency in the Root Apical Meristem of Lycophytes, Non-Seed Vascular Plants, using EdU Labeling.- Osmotic Treatment for Quantifying Cell WallElasticity in the Sepal of Arabidopsis thaliana.- Mapping a Transcriptome Guided Arabidopsis SAM Interactome.- 3D Analysis of Mitosis Distribution Pattern in the Plant Root Tip with iRoCS Toolbox.- Micropropagation of Rosaceous Species SAM Grown in Temperate Climate.- A New Perspective on Cryotherapy: Pathogen Elimination using Plant Shoot Apical Meristem via Cryogenic Techniques.
