Optimization in HPLC - Concepts and Strategies

This handy guide provides detailed practical information for HPLC practitioners of all skill levels, enabling them to optimize the performace of their HPLC/UHPLC system for a wide range of analytical tasks.

List of contents

PART 1: OPTIMIZATION STRATEGIES FOR DIFFERENT MODES AND USES OF HPLC
Working under regulatory constraints
Reverse phase gradient methods
2-Dimensional chromatography
HILIC
LC-MS
Biomolecules
Supercritical fluid chromatography
Chiral substances
Column selection for small molecules
Optimization based on analyte structure
 
PART 2: COMPUTER-AIDED OPTIMIZATION
ChromSword
DryLab
Fusion QbD
Quality by Design approaches to determine the robust range
 
PART 3: CURRENT CHALLENGES FOR HPLC USERS IN INDUSTRY
Big Chemistry
Big Analytical Service Providers
Small Analytical Service Providers
 
PART 4: CURRENT CHALLENGES FOR HPLC EQUIPMENT SUPPLIERS
Thermo Fisher Scientific
Agilent
Shimadzu
Waters
Knauer

About the author

Stavros Kromidas, PhD, works as an independent consultant for analytical chemistry, based in Saarbrucken (Germany). For more than 20 years he has regularly held lectures and training courses on HPLC, and has authored numerous articles and several books on various aspects of chromatography. He is the founder of NOVIA GmbH, a provider of professional training and consulting in analytical chemistry, and served as its CEO until 2001.

Summary

This handy guide provides detailed practical information for HPLC practitioners of all skill levels, enabling them to optimize the performace of their HPLC/UHPLC system for a wide range of analytical tasks.

Product details

• Authors: Stavros Kromidas
• Assisted by: Stavro Kromidas (Editor), Stavros Kromidas (Editor)
• Publisher: Wiley-VCH
• Languages: English
• Product format: Paperback / Softback
• Released: 01.09.2021
• EAN: 9783527347896
• ISBN: 978-3-527-34789-6
• No. of pages: 398
• Dimensions: 172 mm x 20 mm x 245 mm
• Weight: 774 g
• Subjects: Natural sciences, medicine, IT, technology > Chemistry; Non-fiction book > Nature, technology > Natural science; Chemie, Chromatographie, chemistry, Industrial Chemistry, Technische u. Industrielle Chemie, Pharmaceutical & Medicinal Chemistry, Pharmazeutische u. Medizinische Chemie, Chromatographie / Trennverfahren, Chromatography / Separation Techniques, Flüssigkeitschromatographie, HPLC