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This volume details commonly used molecular and cellular techniques and specialized methodologies for studying retina neuronal subtypes and electrophysiology. Chapters describe techniques for anatomical studies of retinal ganglion cell morphology, gap-junction-mediated neuronal connection, multi-electrode array recording on mouse retinas, and paired recording to study the electrical coupling between photoreceptors. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Retinal Development: Methods and Protocols aims to provide readers with a set of practical experimental tools to study retinal development, regeneration, and function of mature retinal neurons. Many of the protocols and strategies described in one organism can be easily adapted to applications in different model systems.
List of contents
Investigating Neurogenesis in Birds.- Studying in vivo Retinal Progenitor Cell Proliferation in Xenopus laevis.- Three-dimensional Culture of Mouse Eyecups.- Live Imaging of Mouse Retinal Slices.- Retinal Strip Culture for Studying Ganglion Cell Axon Growth.- Multiple Approaches for Enhancing Neural Activity to Promote Neurite Outgrowth of Retinal Explants.- Adeno-associate Virus as Gene Delivery Vehicle into Retina.- Transposon-mediated Stable Suppression of Gene Expression in the Developing Chick Retina.- A Simple Guide for Generating BAC Transgenic Animals for Retinal Research.- Identification and Characterization of Cis-regulatory Elements for Photoreceptor Type-specific Transcription in Zebrafish.- Ultrasensitive RNAscope In Situ Hybridization System on Embryonic and Adult Mouse Retinas.- Single Cell Capture, RNA-seq and Transcriptome Analysis from the Neural Retina.- Genetically Directed Sparse Labeling System for Anatomical Studies of Retinal Ganglion Cells.- Intracellular Dye Microinjection in Retinal Morphological and Communication Studies.- Multi-electrode Array Recording of Mouse Retinas Transplanted with Stem-cell Derived Retinal Sheets.- Paired Recording to Study Electrical Coupling between Photoreceptors in Mouse Retina.
