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This volume details key protocols for developing strategies in immunotherapy. Chapters guide the readers through protocols related to various DNA, RNA and protein methods to reprogram the immune system, immune cells, analyzing the effect of the reprogrammed cells, and key methods to consider and analyze patients enrolled in clinical trials with novel immunotherapy regimens. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Cell Reprogramming for Immunotherapy: Methods and Protocols aims to ensure successful results in the further study of this vital field.
List of contents
T Cell Reprogramming against Cancer.- Identification of Cell Surface Targets for CAR T Cell Immunotherapy.- Microfluidic Approach for Highly Efficient Viral Transduction.- Single-cell Cytokine Analysis to Characterize CAR-T Cell Activation.- Testing the Specificity of Compounds Designed to Inhibit CPT1A in T Cells.- Engineering of Natural Killer Cells for Clinical Application.- Dextran Enhances the Lentiviral Transduction Efficiency of Murine and Human Primary NK Cells.- In vivo Assessment of NK Cell-Mediated Cytotoxicity by Adoptively Transferred Splenocyte Rejection.- Immunomodulation of NK Cell Activity.- An Overview of Advances in Cell-based Cancer Immunotherapies Based on the Multiple Immune-cancer Cell Interactions.- Rapid Production of Physiologic Dendritic Cells (phDC) for Immunotherapy.- Reprogramming Exosomes for Immunotherapy.- Nanoparticles for Immune Cell Reprogramming and Reengineering of Tumor Microenvironment.- CRISPR/Cas9 Gene Targeting in Primary Mouse Bone Marrow Derived Macrophages.- Genome-Wide CRISPRi/a Screening in an In Vitro Co-culture Assay of Human Immune Cell with Tumor Cells.- The Propagation and Quantification of Two Emerging Oncolytic Viruses: Vesicular Stomatitis (VSV) and Zika (ZIKV).- Radiolabeling and Imaging of Adoptively Transferred Immune Cells by Positron Emission Tomography.- Functional Analysis of Human Hematopoietic Stem Cells in vivo in Humanized Mice.- Monitoring Allogeneic CAR-T Cells using Flow Cytometry.- Key Factors in Clinical Protocols for Adoptive Cell Therapy in Melanoma.- Place of Academic GMP Facilities in Modern Cell Therapy.
