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This second edition volume expands on the previous edition with a look at the latest techniques used to study plant hormone jasmonate (JA). The chapters in this book and are organized into three parts: Parts One and Two discuss the role of JA in plant physiology and development, and in plant-biotic interactions. Part Three talks about methods used by researchers to study jasmonate metabolism and signaling. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and practical, Jasmonate in Plant Biology: Methods and Protocols, Second Edition is a valuable resource for both novice and expert researchers who are interested in learning more about this developing field.
List of contents
Multiple Roles of Jasmonates in Shaping Rhizotaxis: Emerging Integrators.- Phenotyping of Light Response on JA Defective Mutant in Rice.- Techniques to Determine Effects of Jasmonates on Root Hydraulic Conductivity.- The Role of Jasmonate in Beneficial Microbe-Root Interactions.- Plant-Insect Bioassay for Testing Arabidopsis Resistance to the Generalist Herbivore Spodoptera littoralis.- Targeted Metabolomics of Plant Hormones and Redox Metabolites in Stomatal Immunity.- Bioassays to Evaluate the Resistance of Whole Plants to the Herbivorous Insect Thrips.- Role of Jasmonate in Modulation of Mycorrhizae Induced Resistance against Fungal Pathogens.- Establishment of Actinorhizal Symbiosis in Response to Ethylene, Salicylic Acid, and Jasmonate.- Crystallization of a Complex between MYC and Jas Motif.- A Fluorescence Anisotropy-Based Comprehensive Method for the In Vitro Screening of COI1-JAZs Agonists and Antagonists.- Use of Fluorescent Reporters to Analyse Dynamic and Spatial Responses to Mechanical Wounding.- Quantitative Jasmonate Profiling using a High-Throughput UPLC-nanoESI-MS/MS Method.- Detection of Fungal Jasmonates by Liquid Chromatography Paired with Mass Spectrometry.- CRISPR/Cas9-Mediated Gene Editing of the Jasmonate Biosynthesis OsAOC Gene in Rice.- Mass Spectrometric Approaches to Study the Metabolism of Jasmonates: Biotransformation of Exogenously-Supplemented Methyl Jasmonate by Cell Suspension Cultures of Moringa oleifera.
