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This third edition volume expands on the previous editions with updated approaches and techniques used to study protein nanotechnology and the future of nanomaterial compositions. This book is organized into Three Parts: Part One looks at recombinant protein expression in insect cells, and methods to produce molecular motors, molecular superglues, and protein templates; Part Two explores functionalization strategies and ways to incorporate functional protein components into nanodevices; Part Three discusses various instrumental techniques used to study protein nanostructures. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Protein Nanotechnology: Protocols, Instrumentation,and Applications, Third Edition is a valuable resource for any researchers looking to expand their knowledge in this evolving field.
List of contents
Introduction to Protein Nanotechnology.- Protein Expression in the Baculovirus-Insect Cell Expression System.- Peroxiredoxin Proteins as Building Blocks for Nanotechnology.- Formation of Amphipathic Monolayers from Fungal Hydrophobin Proteins.- ATP Synthase: Expression, Purification, and Function.- Molecular Superglues: Discovery and Engineering Orthogonalization.- Production of Multicomponent Protein Templates for the Positioning and Stabilization of Enzymes.- Adding Function to Protein Scaffolds.- Virus-Derived Nanoparticles.- Strategies for Increasing Protein Stability.- Generation of High Affinity Molecularly Imprinted Nanoparticles for Protein Recognition via a Solid-Phase Synthesis Protocol.- Nanotechnology with S-Layer Proteins.- Preparation of Proteins and Macromolecular Assemblies for Cryo-Electron Microscopy.- Atomic Force Microscopy of Proteins.- Native Protein Mass Spectrometry.- Nanoparticle Tracking Analysis of b-Casein Nanocarriers.- Molecular Dynamics Simulation of Proteins.
