Fr. 192.00

Methicillin-Resistant Staphylococcus Aureus (MRSA) Protocols - Cutting-Edge Technologies and Advancements

English · Paperback / Softback

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Description

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This third edition volume expands on the previous editions with an update on the latest techniques used for the detection, genotyping, and investigating pathogenesis of Staphylococcus aureus in vitro and in vivo. The methods covered in this book mostly focus on routine clinical diagnosis, surveillance, research, and practice for treatment of patients infected by multi-drug resistant S. aureus. The book also covers the epidemiology of MRSA, molecular typing approaches, clinical treatment of MRSA infections, and animal models of drug discovery. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Informative and cutting-edge, Methicillin-Resistant Staphylococcus Aureus (MRSA) Protocols: Cutting-Edge Technologies and Advancements, Third Edition is a valuable resource for researchers looking to set up new methods to study S. aureus, and will also be very useful for technicians and scientists working on other bacterial pathogens. 

List of contents

Clinical, Epidemiologic, and Laboratory Aspects of Methicillin-Resistant Staphylococcus aureus Infections.- Rapid Methods for Detection of MRSA in Clinical Specimens.- Immunofluorescence Microscopy for the Detection of Surface Antigens in Methicillin Resistant Staphylococcus aureus.- Staphylococcal Cassette Chromosome mec (SCCmec) Analysis of MRSA.- Pulsed-Field Gel Electrophoresis Typing of Staphylococcus aureus Strains.- Spa Genotyping of Staphylococcus aureus Isolates.- Multilocus Sequence Typing of Staphylococcus aureus.- Genetic Manipulations of Staphylococcal Chromosomal DNA.- Genetic Manipulation of MRSA using CRISPR/Cas9 Technology.-  RNA-seq Identification of Target Genes Mediated by Regulators of Staphylococcus aureus.- Application of Two-Dimensional Difference Gel Electrophoresis in Identification of Factors Responsible for Virulence of Staphylococcus aureus.- Identification of Virulence Determinants during Host-Pathogen Interaction using Tn-Seq Technology.- Metabolomic Profiling of Staphylococcus aureus.- Determining Impact of Growth Phases on Capacity of Staphylococcus aureus to Adhere to and Invade Host Cells.- Preclinical Models and Methodologies for Monitoring Staphylococcus aureus Infections using Noninvasive Optical Imaging.- Methicillin-Resistant Staphylococcus aureus Infection and Treatment Options.- Animal Models for Drug Development for MRSA.

Report

"The books in the Methods in Molecular Biology series are written for predoctoral and postdoctoral trainees and other scientists who are actively engaged in the design of experiments in the laboratory." (Paul D Fey, Doody's Book Reviews, March 20, 2020)

Product details

Assisted by Yindu Ji (Editor), Yinduo Ji (Editor)
Publisher Springer, Berlin
 
Languages English
Product format Paperback / Softback
Released 01.09.2020
 
EAN 9781493998517
ISBN 978-1-4939-9851-7
No. of pages 268
Dimensions 179 mm x 16 mm x 254 mm
Illustrations XI, 268 p. 35 illus., 27 illus. in color.
Series Methods in Molecular Biology
Subject Natural sciences, medicine, IT, technology > Biology > General, dictionaries

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