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This third edition provides in-depth knowledge on the delivery of naked DNA and small-interfering RNA (siRNA) to the targeted microorganism, mammalian single cells, tissues, and animals for prevention and treatment of disease. It builds on the success of the first edition and on the progress made in siRNA delivery and DNA vaccines for large animals as well as discovery of electroporation applications for the fragile tissues and for internal organs. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, Electroporation Protocols: Microorganism, Mammalian System, and Nanodevice, Third Edition aims to be an invaluable resource for investigators both in and outside of this field.
List of contents
Cell Size-specific Transfection by Micropillar Array Electroporation.- Soft Electroporation through 3D Hollow Nanoelectrodes.- Micromachining of Polymeric Microfluidic Micro-/Nano-Electroporation Device.- 3D Nanochannel Array for High-Throughput Cell Manipulation and Electroporation.- Low-Voltage Flow-Through Electroporation Membrane and Method.- Nanofountain Probe Electroporation for Monoclonal Cell Line Generation.- 3D Nanochannel Electroporation for Macromolecular Nucleotides Delivery.- Nanoelectroporation and Collection of Genetically Modified Exosomes in Primary Cultures of Dendritic Cells.- Electroporation of CRISPR-Cas9 into Malignant B Cells for Loss-of-function Studies of Target Gene via Knockout.- Microfluidic Device for Localized Electroporation.- Transdermal Delivery of Nucleic Acid Mediated by Punching and Electroporation.- Targeted in vivo Electroporation Using Nanoengineered Microelectrodes.- Zygote Electroporation for CRISPR/Cas9 Delivery to Generate Genetically Modified Mice.- Genome Editing in Staphylococcus aureus by Conditional Recombineering and CRISPR/Cas9-mediated Counterselection.- Tissue Nano-Transfection of Surgically Exposed Stroma.- Transformation of a Model Microalga, Chlamydomonas reinhardtii without Cell-wall Removal.- Electroporation Transformation Protocol for Phaeodactylum tricornutum.- Efficient Transformation of the Diatoms Phaeodactylum tricornutum by Multi-pulse Electroporation.- Rapid and Effective Electroporation Protocol for Nannochloropsis oceanica.- Electroporation Procedures for Genetic Modification of Green Algae Chlorella spp..- Electrotransformation of Saccharomyces cerevisiae.
