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This volume explores the latest synthetic procedures for producing receptor-specific retinoids and rexinoids, molecular biology methods, and new technologies to demonstrate the therapeutic activities of molecules. The chapters in this book cover topics such as lentiviral delivery of shRNA constructs into acute promyelocytic leukemia cells for ATRA induced differentiation and autophagy; methods to analyze RAR signaling in colorectal cancer cells; differentiation of primary myoblasts by using RXR agonist; methodology for analyzing effects of retinoid treatment on nervous system development and larval swimming behavior; and protocols for assessment of autophagic flux in ATRA treated 2D and 3D breast cancer cultures. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshootingand avoiding known pitfalls.
Cutting-edge and comprehensive, Retinoid and Rexinoid Signaling: Methods and Protocols is a valuable resources for graduate students, postdoctoral fellows, and principal investigators who are interested in further exploring the signaling mechanisms of these molecules in their specific preclinical models.
List of contents
Proteomics to Predict Loss of RXR-Gamma During Progression of Epithelial Ovarian Cancer.- Targeting Non-Genomic Activity of Retinoic Acid Receptor-Gamma by Acacetin.- Lentiviral Mediated shRNA Approaches: Applications in Cellular Differentiation and Autophagy.- Ligand Design for Modulation of RXR Functions.- In Vitro Models to Study the Regulatory Roles of Retinoids in Angiogenesis.- Analysis of Retinoic Acid Receptor Signaling in Colorectal Cancer.- Methods to Assess Activity and Potency of Rexinoids using Rapid Luciferase-Based Assays: A Case Study with Net-TMN.- Methods to Generate an Array of Novel Rexinoids by SAR on a Potent Retinoid X Receptor Agonist: A Case Study with Net-TMN.- Promoting Primary Myoblast Differentiation through Retinoid X Receptor Signaling.- Mapping Retinoic Acid-Dependant 5mC Derivatives in Mouse Embryonic Fibroblasts.- Hemodynamics-Based Strategy of Using Retinoic Acid Receptor and Retinoid X Receptor Agonists to Induce MicroRNA-10a and Inhibit Atherosclerotic Lesion.- Evaluating the Role of RARbeta Signaling on Cellular Metabolism in Melanoma Using the Seahorse XF Analyzer.- Highly Sensitive Quantitative Determination of Retinoic Acid Levels, Retinoic Acid Synthesis, and Catabolism in Embryonic Tissue Using a Reporter Cell-Based Method.- A Behavioral Assay to Study Effects of Retinoid Pharmacology on Nervous System Development in a Marine Annelid.- Retinoic Acid as a Modulator of Proximal-Distal Patterning and Branching Morphogenesis of the Avian Lung.- Translation of Chemopreventive Effects of Retinoids and Rexinoids: Extraction and Quality Assessment of RNA form Formalin-Fixed Tissues.- Assessing Autophagy during Retinoid Treatment of Breast Cancer Cells.- Non-Radioactive and Radioactive Telomerase Assays for Detecting Diminished Telomerase Activity in Cancer Cells after Treatment with Retinoid.
