Read more
This volume discusses different enzyme-catalyzed ligation methodologies for a variety of different chemical transformations. This book wants readers to view enzymes as a powerful tool in both academic and industrial research. Chapters in this book cover topics such as sortase A-mediated generation of site-specifically conjugated antibody-drug conjugates; omniligase-catalyzed inter- and intramolecular ligation; ligation catalyzed by microbial transglutaminase; peptide cyclization mediated by cyanobactin macrocyclases, butelase 1 and sortase A; using BioID as a tool for protein proximity labeling in living cells; and inducible, selective labeling of proteins via enzymatic oxidation of tyrosine. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and thorough, Enzyme-Mediated Ligation Methods is a valuable resource for students and scientists from different disciplines who are interested in using enzymatic strategies to answer their research questions.
List of contents
Sortase A Enzyme-Mediated Generation of Site-Specifically Conjugated Antibody-Drug Conjugates.- Site-Specific C-Terminal Labeling of Recombinant Proteins with Proximity-Based Sortase-Mediated Ligation (PBSL).- Cyclizing Disulfide-Rich Peptides using Sortase A.- Chemo-Enzymatic Synthesis of Linear- and Head-to-Tail Cyclic Peptides using Omniligase-1.- Site-Specific Labeling of Proteins using the Formylglycine-Generating Enzyme (FGE).- Butelase 1-Mediated Ligation of Peptides and Proteins.- Trypsiligase-Catalyzed Peptide and Protein Ligation.- Site-Specific Antibody-Drug Conjugation Using Microbial Transglutaminase.- Tailoring Activity and Selectivity of Microbial Transglutaminase.- SpyLigase-Catalyzed Modification of Antibodies.- Peptide Cyclization Catalyzed by Cyanobactin Macrocyclases.- In Vitro and In Planta Cyclization of Target Peptides using an Asparaginyl Endopeptidase from Oldenlandia affinis.- Site-Specific Antibody Labeling Using Phosphopantetheinyl Transferase-Catalyzed Ligation.- Lipoic Acid Ligase-Promoted Bioorthogonal Protein Modification and Immobilization.- BioID as a Tool for Protein Proximity Labeling in Living Cells.- N-Myristoyl transferase (NMT)-Catalyzed Labeling of Bacterial Proteins for Imaging in Fixed and Live Cells.- Tubulin Tyrosine Ligase-Mediated Modification of Proteins.- Inducible, Selective Labeling of Proteins via Enzymatic Oxidation of Tyrosine.
