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This updated collection addresses the intense efforts that have been underway to characterize the cellular-molecular-biochemical elements of not only the hematopoietic stem cell niche but the niches for other stem cells. These specialized microenvironments that regulate stem cell function have continued to inspire tremendous interest as a subject of study in the years since the publication of the first edition. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Stem Cell Niche: Methods and Protocols, Second Edition serves as an ideal guide for both experts and novices in the stem cell field.
List of contents
In Vitro Maintenance of Multipotent Neural Crest Stem Cells as Crestospheres.- Analysis of Hematopoietic Niche in the Mouse Embryo.- Functional Assays of Stem Cell Properties Derived from Different Niches.- Ex Vivo Visualization and Analysis of the Muscle Stem Cell Niche.- Stem Cell-Derived Cardiac Spheroids as 3D In Vitro Models of the Human Heart Microenvironment.- Isolation, Propagation, and Clonogenicity of Intestinal Stem Cells.- Isolation of Extracellular Vesicles from Subventricular Zone Neural Stem Cells.- Reconstruction of Regenerative Stem Cell Niche by Cell Aggregate Engineering.- Three-Dimensional Co-Culture of Human Hematopoietic Stem/Progenitor Cells and Mesenchymal Stem/Stromal Cells in a Biomimetic Hematopoietic Niche Microenvironment.- Investigating the Vascular Niche: Three-Dimensional Co-Culture of Human Skeletal Muscle Stem Cells and Endothelial Cells.- Identification and Characterization of Stem Cells in Oral Cancer.- Determining Competitive Potential of Bone Metastatic Cancer Cells in the Murine Hematopoietic Stem Cell Niche.- Spatial Genomic Analysis: A Multiplexed Transcriptional Profiling Method that Reveals Subpopulations of Cells within Intact Tissues.- 3D Culture of Mesenchymal Stem Cells in Alginate Hydrogels.- Isolation and Identification of Murine Bone Marrow-Derived Macrophages and Osteomacs from Neonatal and Adult Mice.
