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The volume brings together some of the best experts in the field of modern metabolomics to discuss various techniques used today to study specific metabolite classes, and metabolomics in bacterial systems and mammalian systems. The chapters in this book cover topics such as Isotopic Ratio Outlier Analysis (IROA) for quantitative analysis; cholesterol and derivatives in ocular tissues using LC-MS/MS methods; microbial siderophores analysis by mass spectrometry; the metabolomic study of tissues in Parkinson's Disease; and NMR analysis in livestock metabolomics. Written in the highly successful Methods in Molecular Biology series format, the chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step readily reproducible laboratory protocols, tips on troubleshooting and avoiding pitfalls.
Cutting-edge and thorough, Metabolomics: Methods and Protocols is an essential resource for any researcher interested inthis exciting and evolving field.
List of contents
Collection of Untargeted Metabolomic Data for Mammalian Urine Applying HILIC and Reversed Phase Ultra Performance Liquid Chromatography Methods Coupled to a Q Exactive Mass Spectrometer.- Isotopic Ratio Outlier Analysis (IROA) for Quantitative Analysis.- Nuclear Magnetic Resonance Measurement of Metabolic Flux Using 13C and 1H Signals.- Quantitative Metabolomics using Isotope Residue Outlier Analysis (IROA®) with Internal Standards.- Analyses of Cholesterol Metabolites of Optic Nerve using GC-MS Methods.- Analyses of Cholesterol and Derivatives in Ocular Tissues using LC-MS/MS Methods.- Measurement of Pyridine Nucleotides in Biological Samples using LC-MS/MS.- Handling and Analysis of 5-Formyl-, 5,10-Methenyl-, 10-Formyl-, 5-Formimno-, and 5,10-Methylenetetrahydrofolates.- Protein-Lipid Complex Separation Utilizing a Capillary Electrophoresis System.- Immature and Mature Collagen Crosslinks Quantification using High Performance Liquid Chromatography and High Resolution Mass Spectrometry in OrbitrapTM.- Relative Quantitation of Neurotoxic Metabolites of the Kynurenine Pathway by Laser Desorption Ionization Mass Spectrometry (LDI-MS).- Analysis of Microbial Siderophores by Mass Spectrometry.- HSD18B7 Enzyme Assay Technique using a Triple Quadrupole Mass Spectrometer.- Assays for Intracellular Cyclic Adenosine Monophosphate (cAMP) and Lysosomal Acidification.- Isotopic Ratio Outlier Analysis (IROA) of Aqueous Humor for Metabolites.- Assessment of Transport of Lipid Metabolites within Trabecular Meshwork Cells.- Detection and Quantification of Lipid Droplets in Differentiated Human Podocytes.- Labeling Cancer Stem Cells with 13C6 Glucose and 13C5 Glutamine for Metabolic Flux Analysis.- Protocols for Metabolomic Analyses from Tissues in Parkinson's Disease.- Application of Stable Isotope Labels for Metabolomics in Studies in Fatty Liver Disease.- Proteometabolomics ofMelphalan Resistance in Multiple Myeloma.- Metabolomic Analyses of Muscle Atrophy Induced by Hindlimb Unloading.- Protocols for NMR Analysis in Livestock Metabolomics.- Metabolomics Applicable to Retinal Vascular Diseases.
