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This detailed book explores strategies that have been developed to combat plant virus infection. Beginning with a section on techniques for identifying and studying the virus resistance gene involved in plant innate immunity, the volume continues by delving into techniques related to novel mechanisms of plant virus resistance, methods for the analysis and practical use of RNA silencing, as well as methods for the development of plant viral vaccines. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Antiviral Resistance in Plants: Methods and Protocols serves as an ideal guide for researchers working to combat the serious threats plant virus diseases represent for agricultural production and beyond.
List of contents
Identification and Functional Analysis of NB-LRR-Type Virus Resistance Genes-Overview and Functional Analysis of Candidate Genes.- Insertion of Epitope Tag into NB-LRR Class Plant Virus Resistance Protein.- Reverse genetic analysis of antiviral resistance signaling and the resistance mechanism in Arabidopsis thaliana.- Analysis of Antiviral Resistance Signaling Pathways by Virus-Induced Gene Silencing in Nicotiana benthamiana.- Random Mutagenesis of Virus Gene for the Experimental Evaluation of the Durability of NB-LRR Class Plant Virus Resistance Gene.- A Cell-free Replication System for Positive-strand RNA Viruses for Identification and Characterization of Plant Resistance Gene Products.- Plant Protein-Mediated Inhibition of Virus Cell-to-Cell Movement -Far-western Screening and Biological Analysis of a Plant Protein Interacting with a Viral Movement Protein-.- Transfection of Protoplasts Prepared from Arabidopsis thaliana Leaves for Plant Virus Research.- CRISPR/Cas9-mediated Editing of Genes Encoding rgs-CaM-like Proteins in Transgenic Potato Plants.- A Simplified Method to Engineer CRISPR/Cas9-Mediated Geminivirus Resistance in Plants.- Computational Workflow for Small RNA Profiling in Virus-Infected Plants.- A Sensitized Genetic Screen to Identify Novel Components and Regulators of the Host Antiviral RNA Interference Pathway.- Design, Synthesis and Functional Analysis of Highly Specific Artificial Small RNAs With Antiviral Activity in Plants.- Resistance Breeding through RNA Silencing of Host Factor Involved in Virus Replication.- Large-Scale Inoculation and Evaluation Methods for Attenuated Plant Viruses.- RNA Silencing-Mediated Apple Latent Spherical Virus Vaccine in Plants.
