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This book addresses cutting-edge techniques for researching transposon mutagenesis, an approach for identifying individual gene contributions to the phenotypic characteristics of a particular microorganism. The volume begins with methods for specific microorganisms and include protocols for individual microorganisms ranging from pathogens such as Salmonella to Bifidobacterium, a microorganism considered beneficial to humans and animals. The final section addresses more general protocols including plasmid transfer and bioinformatic tools as well as novel applications of transposon methodologies such as transposon-aided capture of antibiotic resistant plasmids. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Microbial Transposon Mutagenesis: Protocols and Applications serves as a valuable reference for scientists seeking to apply transposon mutagenesis to microbial genetic analyses and functionality.
List of contents
Preparation of Transposon Library and Tn-Seq Amplicon Library for Salmonella Typhimurium.- Application of Whole Genome Sequencing to Transposon Mutants of Salmonella Heidelberg.- Construction of a Sequence-Defined Transposon Mutant Library in Staphylococcus aureus.- Transposon Mutagenesis in Streptococcus Species.- Implementation of Transposon Mutagenesis in Bifidobacterium.- Transposon Mutagenesis of Listeria monocytogenes.- Transposon Mutagenesis of Foodborne Pathogenic Esherichia coli.- Methods for Transposon Mutagenesis in Proteus mirabilis.- Mutagenesis of Vibrio fischeri and Other Marine Bacteria Using Hyperactive Mini-Tn5 Derivatives.- Transposon Mutagenesis of Bacteroides fragilis.- Transposon Mutagenesis in Mycobacterium avium Subspecies Paratuberculosis.- Protocols of Conjugative Plasmid Transfer in Salmonella: Plate, Broth, and Filter Mating Approaches.- Construction of DNA Barcode-Tagged Salmonella Strains.- Transposon-Aided Capture of Antibiotic Resistance Plasmids from Complex Samples.- Efficient Gene Deletion Method for Listeria monocytogenes.- Whole Genome Identification and Characterization of Bacterial Insertion Sequences Using Bioinformatic Tools.
