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This detailed volume explores essential protocols for the study of Proteus mirabilis which, despite its genetic relatedness to species such as E. coli, often requires specialized handling techniques. This opportunistic bacterial pathogen, most often known as a causative agent of complicated urinary tract infection, is addressed in chapters from global experts in the field. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Proteus mirabilis: Methods and Protocols serves as an ideal guide for researchers intrigued by the renewed appreciation for the medical impact and environmental adaptability of this organism, coupled with continued fascination for its dynamic behavior.
List of contents
Proteus mirabilis Overview.- Culture Methods for Proteus mirabilis.- Methods for Studying Swarming and Swimming Motility.- Testing the Ability of Compounds to Induce Swarming.- Purification of Native Flagellin.- Analysis of Proteus mirabilis Social Behaviors on Surfaces.- Insertional Mutagenesis Protocol for Constructing Single or Sequential Mutations.- Allelic Exchange Mutagenesis in Proteus mirabilis.- Quantification of Urease Activity.- Siderophore Detection using Chrome Azurol S and Cross-Feeding Assays.- Using Hemagglutination, Surface Shearing, and Acid Treatment to Study Fimbriae in Proteus mirabilis.- Phase Variation of the mrp Fimbrial Promoter.- Adherence of Proteus mirabilis to Uroepithelial Cells.- An In Vitro Bladder Model for Studying Catheter-Associated Urinary Tract Infection and Associated Analysis of Biofilms.- Independent Transurethral Urinary Tract Inoculation in a Murine Model of Ascending Infection with Proteus mirabilis.- Co-challenge Inoculation with Proteus mirabilis in a Murine Transurethral Urinary Tract Model of Ascending Infection.- Indwelling Urinary Catheter Model of Proteus mirabilis Infection.- Vaccination to Protect against Proteus mirabilis Challenge Utilizing the Ascending Model of Urinary Tract Infection.- Characterization of Proteus mirabilis Lipopolysaccharide Samples by Infrared Spectroscopy and Serological Methods.- Isolation and Purification of Proteus mirabilis Bacteriophage.- Detection of Neutrophil Extracellular Traps in Urine.- Using Proteomics to Identify Inflammation during Urinary Tract Infection.- Assessment of Proteus mirabilis Antigen Immunological Complexes by Atomic Force Microscopy.- Considerations for Modeling Proteus mirabilis Swarming.- Transposon Insertion Site Sequencing in a Urinary Tract Model.
