Molecular Biology - Principles of Virology

Principles of Virology, the leading virology textbook in use, is an extremely valuable and highly informative presentation of virology at the interface of modern cell biology and immunology. This text utilizes a uniquely rational approach by highlighting common principles and processes across all viruses. Using a set of representative viruses to illustrate the breadth of viral complexity, students are able to under-stand viral reproduction and pathogenesis and are equipped with the necessary tools for future encounters with new or understudied viruses.
 
This fifth edition was updated to keep pace with the ever-changing field of virology. In addition to the beloved full-color illustrations, video interviews with leading scientists, movies, and links to exciting blogposts on relevant topics, this edition includes study questions and active learning puzzles in each chapter, as well as short descriptions regarding the key messages of references of special interest.
 
Volume I: Molecular Biology focuses on the molecular processes of viral reproduction, from entry through release. Volume II: Pathogenesis and Control addresses the interplay between viruses and their host organisms, on both the micro- and macroscale, including chapters on public health, the immune response, vaccines and other antiviral strategies, viral evolution, and a brand new chapter on the therapeutic uses of viruses. These two volumes can be used for separate courses or together in a single course. Each includes a unique appendix, glossary, and links to internet resources.
 
Principles of Virology, Fifth Edition, is ideal for teaching the strategies by which all viruses reproduce, spread within a host, and are maintained within populations. This edition carefully reflects the results of extensive vetting and feedback received from course instructors and students, making this renowned textbook even more appropriate for undergraduate and graduate courses in virology, microbiology, and infectious diseases.

List of contents

Preface xvii
 
Acknowledgments xxi
 
About the Authors xxiii
 
Key of Repetitive Elements xxv
 
Part I The Science of Virology 1
 
1 Foundations 2
 
Luria's Credo 3
 
Viruses Defined 3
 
Why We Study Viruses 3
 
Viruses Are Everywhere 3
 
Viruses Infect All Living Things 4
 
Viruses Can Cause Human Disease 5
 
Viruses Can Be Beneficial 5
 
Viruses "R" Us 6
 
Viruses Can Cross Species Boundaries 6
 
Viruses Are Unique Tools To Study Biology 6
 
Virus Prehistory 7
 
Viral Infections in Antiquity 7
 
The First Vaccines 8
 
Microorganisms as Pathogenic Agents 9
 
Discovery of Viruses 11
 
The Defining Properties of Viruses 13
 
The Structural Simplicity of Virus Particles 13
 
The Intracellular Parasitism of Viruses 13
 
Cataloging Animal Viruses 18
 
The Classical System 18
 
Classification by Genome Type: the Baltimore System 19
 
A Common Strategy for Viral Propagation 21
 
Perspectives 21
 
References 24
 
Study Questions 24
 
2 The Infectious Cycle 26
 
Introduction 27
 
The Infectious Cycle 27
 
The Cell 27
 
Entering Cells 28
 
Viral RNA Synthesis 29
 
Viral Protein Synthesis 29
 
Viral Genome Replication 29
 
Assembly of Progeny Virus Particles 29
 
Viral Pathogenesis 29
 
Overcoming Host Defenses 30
 
Cultivation of Viruses 30
 
Cell Culture 30
 
Embryonated Eggs 35
 
Laboratory Animals 35
 
Assay of Viruses 35
 
Measurement of Infectious Units 35
 
Efficiency of Plating 38
 
Measurement of Virus Particles 40
 
Viral Reproduction: The Burst Concept 49
 
The One-Step Growth Cycle 49
 
One-Step Growth Analysis: a Valuable Tool for Studying Animal Viruses 52
 
Global Analysis 53
 
DNA Microarrays 54
 
Mass Spectrometry 56
 
Protein-Protein Interactions 56
 
Single-Cell
 
Virology 56
 
Perspectives 58
 
References 59
 
Study Questions 60
 
Part II Molecular Biology 61
 
3 Genomes and Genetics 62
 
Introduction 63
 
Genome Principles and the Baltimore System 63
 
Structure and Complexity of Viral Genomes 63
 
DNA Genomes 64
 
RNA Genomes 65
 
What Do Viral Genomes Look Like? 68
 
Coding Strategies 69
 
What Can Viral Sequences Tell Us? 69
 
The "Big and Small" of Viral Genomes: Does Size Matter? 71
 
The Origin of Viral Genomes 73
 
Genetic Analysis of Viruses 74
 
Classical Genetic Methods 75
 
Engineering Mutations into Viral Genomes 77
 
Engineering Viral Genomes: Viral Vectors 83
 
Perspectives 87
 
References 87
 
Study Questions 88
 
4 Structure 90
 
Introduction 91
 
Functions of the Virion 91
 
Nomenclature 92
 
Methods for Studying Virus Structure 92
 
Building a Protective Coat 95
 
Helical Structures 96
 
Capsids with Icosahedral Symmetry 99
 
Other Capsid Architectures 111
 
Packaging the Nucleic Acid Genome 112
 
Direct Contact of the Genome with a Protein Shell 112
 
Packaging by Specialized Viral Proteins 113
 
Packaging by Cellular Proteins 113
 
Viruses with Envelopes 115
 
Viral Envelope Components 115
 
Simple Enveloped Viruses: Direct Contact of External Proteins with the

About the author

Jane Flint is Professor Emerita of Molecular Biology at Princeton University. Dr. Flint's research focused on investigation of the mechanisms by which viral gene products modulate host pathways and antiviral defenses to allow efficient reproduction in normal human cells of adenoviruses, viruses that are used in such therapeutic applications as gene transfer and cancer treatment.
 
Vincent R. Racaniello is Higgins Professor of Microbiology & Immunology at Columbia University Vagelos College of Physicians & Surgeons. Dr. Racaniello has been studying viruses for over 40 years, including polio- virus, rhinovirus, enteroviruses, hepatitis C virus, and Zika virus. He blogs about virus-es at virology.ws and is host of This Week in Virology.
 
Glenn F. Rall is a Professor and the Chief Academic Officer at the Fox Chase Cancer Center, and is an Adjunct Professor in the Microbiology and Immunology departments at the University of Pennsylvania, as well as Thomas Jefferson, Drexel, and Temple Universities. Dr. Rall studies viral infections of the brain and the immune responses to those infections, with the goal of defining how viruses contribute to disease.
 
Theodora Hatziioannou is a Research Associate Professor at Rockefeller University and is actively involved in teaching programs at Albert Einstein College of Medicine. Dr. Hatziioannou has worked on multiple viruses with a focus on retroviruses and the molecular mechanisms that govern virus tropism and on the improvement of animal models for human disease.
 
Anna Marie Skalka is a Professor Emerita and former Senior Vice President for Basic Research at the Fox Chase Cancer Center. Dr. Skalka is internationally recognized for her contributions to the understanding of the biochemical mechanisms by which retroviruses replicate and insert their genetic material into the host genome, as well as her research into other molecular aspects of retrovirus biology.

Summary

Principles of Virology, the leading virology textbook in use, is an extremely valuable and highly informative presentation of virology at the interface of modern cell biology and immunology. This text utilizes a uniquely rational approach by highlighting common principles and processes across all viruses. Using a set of representative viruses to illustrate the breadth of viral complexity, students are able to under-stand viral reproduction and pathogenesis and are equipped with the necessary tools for future encounters with new or understudied viruses.

This fifth edition was updated to keep pace with the ever-changing field of virology. In addition to the beloved full-color illustrations, video interviews with leading scientists, movies, and links to exciting blogposts on relevant topics, this edition includes study questions and active learning puzzles in each chapter, as well as short descriptions regarding the key messages of references of special interest.

Volume I: Molecular Biology focuses on the molecular processes of viral reproduction, from entry through release. Volume II: Pathogenesis and Control addresses the interplay between viruses and their host organisms, on both the micro- and macroscale, including chapters on public health, the immune response, vaccines and other antiviral strategies, viral evolution, and a brand new chapter on the therapeutic uses of viruses. These two volumes can be used for separate courses or together in a single course. Each includes a unique appendix, glossary, and links to internet resources.

Principles of Virology, Fifth Edition, is ideal for teaching the strategies by which all viruses reproduce, spread within a host, and are maintained within populations. This edition carefully reflects the results of extensive vetting and feedback received from course instructors and students, making this renowned textbook even more appropriate for undergraduate and graduate courses in virology, microbiology, and infectious diseases.

Product details

• Authors: Jan Flint, Jane Flint, Theodora Hatziioannou, Vincent Racaniello, Vincent R Racaniello, Vincent R. Racaniello, Glenn F et Rall, Glenn F. Rall, Anna Marie Skalka
• Publisher: American Society for Microbiology
• Languages: English
• Product format: Paperback / Softback
• Released: 14.09.2020
• EAN: 9781683672845
• ISBN: 978-1-68367-284-5
• Dimensions: 220 mm x 280 mm x 27 mm
• Series: The Teacher's Toolbox Series; ASM
• Subjects: Natural sciences, medicine, IT, technology; Mikrobiologie, Virologie, Molekularbiologie, Life Sciences, Biowissenschaften, Virology, Mikrobiologie u. Virologie, Microbiology & Virology