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This volume looks at a collection of stem cell and regenerative techniques used by both novice and expert scientists. Chapters cover topics such as tissue repaired by expansion and reprogramming; induced pluripotent stem cells driven in neuronal or vascular differentiation; using mesenchymal stem cells to derive skeletal muscle, osteoblasts, and spermatogonial cells; and the technique of monitoring the development of sub-organ microenvironments in the developing pancreas. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and thorough, Stem Cells and Tissue Repair: Methods and Protocols, Second Edition is a valuable resource that provides readers with the latest descriptions and references for exploring this vast field in regenerative medicine.
List of contents
Culturing and Manipulating Mouse Embryonic Stem Cells.- Generation of an Induced Pluripotent Stem Cell Line with the Constitutive EGFP Reporter.- Engraftable Induced Pluripotent Stem Cell-Derived Neural Precursors for Brain Repair.- In Vitro Direct Reprogramming of Mouse and Human Astrocytes to Induced Neurons.- Preparation of Bioscaffolds Delivering Stem Cells for Neural Regeneration.- Improved Isolation of Human Vascular Wall-Resident Mesenchymal Stem Cells.- In Vitro Generation of Vascular Wall-Typical Mesenchymal Stem Cells (VW-MSC) from Murine Induced Pluripotent Stem Cells through VW-MSC Specific Gene Transfer.- Analysis of Tooth Innervation in Microfluidic Co-Culture Devices.- Peripheral Nerve Regeneration in a Novel Rat Model of Dysphagia.- Healing of Full Thickness Murine Skin Wounds Containing Nanofibers using Splints for Efficient Re-Epithelialization and to Avoid Contracture.- Enrichment and Characterization of Human and Murine Pulmonary Mesenchymal Progenitor Cells (MPC).- Isolation and Culture of Quiescent Skeletal Muscle Satellite Cells.- Isolation, Culture, Cryopreservation and Identification of Bovine, Murine, and Human Spermatogonial Stem Cells.- Long-Term Ex Vivo Expansion of Murine Spermatogonial Stem Cells in a Simple Serum-Free Medium.- Generating Kidney Organoids from Human Pluripotent Stem Cells Using Defined Conditions.- Painting the Pancreas in Three Dimensions: Whole Mount Immunofluorescence Method.- Induction of Osteoblasts by Direct Reprogramming of Mouse Fibroblasts.
