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Part 1 of this volume reviews advances in gene editing techniques such as insertion-based genome edits, base editing, guide RNAs and CRISPR/Cas off targeting. Part 2 surveys applications of gene editing in key cereal and vegetable crops.
List of contents
Part 1 Genome editing techniques1.Using TALENS and zinc finger nucleases as genome-editing reagents in plants:
Adam Bogdanove, Cornell University, USA;
2.Double-strand break (DSB) repair in plants:
Holger Puchta, Botanical Institute II - Karlsruhe Institute of Technology, Germany;
3.Advances in the generation of insertion-based genome edits in plants:
Qing-Hui Yu, Xinjiang Chinese Academy of Agricultural Sciences, China;
4.Viruses as vectors for the delivery of gene-editing reagents:
Evan E. Ellison, James C. Chamness and Daniel F. Voytas, University of Minnesota, USA;
5.Base editing in plants:
Nathaniel Graham, Pairwise Plant, USA;
6.Advances in designing guide RNAs in genome editing of plants:
Ling-Ling Chen, Huazhong Agricultural University, China;
7.Advances in assembling gRNA/Cas9 constructs in genome editing of plants:
Diego Orzaez, Polytechnic University of Valencia, Spain;
8.Strategies for CRISPR/Cas9-mediated genome editing: from delivery to production of modified plants:
William Gordon-Kamm, Pierluigi Barone, Sergei Svitashev, Jeffry D. Sander, Sandeep Kumar and Todd Jones, Corteva Agriscience, USA;
9.Advances in screening plants for edits and off targets:
Chung Wang, Chinese Academy of Agricultural Sciences, China;
10.Genome editing on promoter regions:
Andika Gunadi and Ning Zhang, Boyce Thompson Institute, USA; and John J. Finer, The Ohio State University, USA;
11.The regulation of genome-edited crops:
Gregory Jaffe, Center for Science in the Public Interest, USA;
Part 2 Applications12.Genome editing of barley:
Martin Becker, Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Germany; and Goetz Hensel, Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Germany and Palacký University, Czech Republic;
13.Genome editing of maize:
Kan Wang, Iowa State University, USA;
14.Genome editing of sorghum:
David Holding, University of Nebraska-Lincoln, USA;
15.Genome editing of brassica crops:
Cheng Dai, Huazhong Agricultural University, China;
16.Genome editing of tomatoes and other Solanaceae:
Joyce Van Eck, Boyce Thompson Institute - Cornell University, USA;
17.Genome editing of perennial crops:
Chung Jui (CJ) Tsai, University of Georgia, USA;
Summary
Part 1 of this volume reviews advances in gene editing techniques such as insertion-based genome edits, base editing, guide RNAs and CRISPR/Cas off targeting. Part 2 surveys applications of gene editing in key cereal and vegetable crops.
