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This third edition aims to provide new and updated methods on animal influenza viruses as well as more advanced protocols that will guide the reader in designing research. Chapters detail influenza in peridomestic animals, marine mammals, savian influenza, swine influenza, equine influenza, hemagglutination, genome sequencing, and influenza in other mammals. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Animal Influenza Virus: Methods and Protocols, Third Edition aims to ensure successful results in the further study of this vital field.
List of contents
Hemagglutination Assay for Influenza Virus.- Hemagglutination Inhibition Assay.- Immunohistochemical Staining of Influenza Virus in Tissues.- Plasmid-based Reverse Genetics of Influenza A virus.- Antigenic Cartography: Overview and Current Developments.- Complete Genome Sequencing of Influenza A viruses using Next-Ggeneration Sequencing.- A Brief Introduction to Avian Influenza Virus.- Wild Bird Surveillance for Avian Influenza Virus.- Avian Influenza Virus Sample Types, Collection, and Handling.- Avian Influenza Virus RNA Extraction.- Avian Influenza Virus Detection and Quantitation by Real-time RT-PCR.- Avian Influenza Virus Isolation, Propagation, and Titration in Embryonated Chicken Eggs.- Agar Gel Immunodiffusion Assay to Detect Antibodies to Type A Influenza Virus.- Detection of Influenza A Antibodies in Avian Samples by ELISA.- Conducting Influenza Virus Pathogenesis Studies in Avian Species.- Laboratory Methods for Assessing and Licensing Influenza Vaccines for Poultry.- Techniques for the Measurement of Cell Mediated Immune Responses to Avian Influenza Virus.- A Brief Introduction to Influenza A Virus in Swine.- Specimen Types, Collection, and Transport for Influenza A Viruses of Swine.- Isolation of Swine Influenza A Virus in Cell Cultures and Embryonated Chicken Eggs.- RNA Extraction from Swine Samples and Detection of Influenza A Virus in Swine by Real-time RT-PCR.- Enzyme-linked Immunosorbent Assay for Detection of Serum or Mucosal Isotype SpecificIgG and IgA Whole Virus Antibody to Influenza A virus in Swine.- Serum Virus Neutralization Assay for Detection and Quantitation of Serum Neutralizing Antibodies to Influenza A Virus in Swine.- Detection and Titration of Influenza A Virus Neuraminidase Inhibiting (NAI) Antibodies using an Enzyme-Linked Lectin Assay (ELLA).- In vivo Models for Pathotyping and Vaccine Efficacy for Swine Influenza.- A Brief Introduction to Equine Influenza and Equine Influenza Viruses.- Equine Influenza Diagnosis: Sample Collection and Transport.- RNA Extraction from Equine Samples for Equine Influenza Virus.- Type A Influenza Virus Detection from Horses by Real-Time RT-qPCR and Insulated Isothermal RT-PCR.- Equine Influenza Culture Methods.- Equine Influenza Serological Methods.- Influenza A Viruses in Peridomestic Mammals.- A Brief Introduction to Influenza A Virus in Marine Mammals.
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