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This book illustrates experimental and computational methodologies used to achieve cost effective biological processes for the production of fuels and biochemicals through multiple approaches to increasing yield, titers, and productivity in a robust host. The volume includes the most recent and cutting-edge aspects of pathway engineering, flux analysis, and metabolic enzyme engineering. Each chapter highlights the complexity and challenges of the problem as well as the methods used to solve this problem or changes needed in current methods. As a part of the highly successful Methods in Molecular Biology series, chapters include the kind of detailed implementation advice that gives researchers a much needed boost.
Authoritative and practical, Metabolic Pathway Engineering benefits not only scientists working on more fundamental aspects of this endeavor but also those in the biochemical industry working on strain engineering for robust industrial processes.
List of contents
Overview and Future Directions.- Genetics of Unstudied Thermophiles for Industry.- Methods for Metabolic Engineering of Thermoanaerobacterium saccharolyticum.- Methods for Metabolic Engineering of a Filamentous Trichoderma reesei.- Methods for Algal Protein Isolation and Proteome Analysis.- An Improved Leaf Protoplast System for Highly Efficient Transient Expression in Switchgrass (Panicum virgatum L.).- Characterizing Intracellular Proteomes for Microbes: An Experimental Approach Using Label-Free Protein Quantitation.- Bacterial Differential Expression Analysis Methods.- Measuring Biomass-Derived Products in Biological Conversion and Metabolic Process.- Crystallography of Metabolic Enzymes.- Measuring Metabolic Enzyme Performance.- Gene Editing Technologies for Biofuel Production in Thermophilic Microbes.- Software and Methods for Computational Flux Balance Analysis.- Dynamic Flux Analysis: An Experimental Approach of Fluxomics.- Network Modeling of Complex Data Sets.- Connecting Microbial Genotype with Phenotype in the Omics Era.
