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This second edition volume expands on the previous edition with a look at the latest techniques in plant epigenetics and epigenomic research. Chapters in this book cover topics such as whole genome methylome analysis; analysis of DNA methylation; chromatin analysis of metabolic gene clusters in plants; plant epigenetic stress memory induced by drought; small non-coding RNA analysis in seeds of forest tree species; and a discussion on epigenetics inheritance in plants. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Plant Epigenetics and Epigenomics: Methods and Protocols, Second Edition is a valuable resource for both novice and expert researchers, and aims to advance this field of study by paving the way for integrating epigenetic mechanisms into models of plant function during development and evolution.
List of contents
An Overview of Current Research in Plant Epigenetic and Epigenomic Phenomena.- Approaches to Whole Genome Methylome Analysis in Plants.- Understanding DNA Methylation Patterns in Wheat.- MCSeEd (Methylation Context Sensitive Enzyme ddRAD): A New Method to Analyze DNA Methylation.- Plant-RRBS: DNA Methylome Profiling Adjusted to Plant Genomes, Utilizing Efficient Endonuclease Combinations, for Multi-Sample Studies.- Rice Histone Propionylation and Generation of Chemically Derivatized Synthetic H3 and H4 Peptides for Identification of Acetylation Sites and Quantification.- Preparing Chromatin and RNA from Rare Cell Types with Fluorescence-Activated Nuclear Sorting (FANS).- Measurement of Arabidopsis thaliana Nuclear Size and Shape.- Study of Cell-Type-Specific Chromatin Organization: In Situ Hi-C Library Preparation for Low-Input Plant Materials.- Chromatin Analysis of Metabolic Gene Clusters in Plants.- Characterization of Plant 3DChromatin Architecture, In Situ Hi-C Library Preparation and Data Analysis.- The Gene Balance Hypothesis: Epigenetics and Dosage Effects in Plants.- Identification and Comparison of Imprinted Genes across Plant Species.- Epigenetic Approaches in Non-Model Plants.- Techniques for Small Non-Coding RNA Analysis in Seeds of Forest Tree Species.- Epigenetic Barcodes for Detection of Adulterated Plants and Plant-Derived Products.- Plant Epigenetic Stress Memory Induced by Drought: A Physiological and Molecular Perspective.- A Critical Guide for Studies on Epigenetic Inheritance in Plants.
