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This open access book presents simple, robust pre-field screening protocols that allow plant breeders to screen for enhanced tolerance to heat stress in rice. Two critical heat-sensitive stages in the lifecycle of the rice crop are targeted - the seedling and flowering stages - with screening based on simple phenotypic responses. The protocols are based on the use of a hydroponics system and/or pot experiments in a glasshouse in combination with a controlled growth chamber where the heat stress treatment is applied. The protocols are designed to be effective, simple, reproducible and user-friendly.
The protocols will enable plant breeders to effectively reduce the number of plants from a few thousands to less than 100 candidate individual mutants or lines in a greenhouse/growth chamber, which can then be used for further testing and validation in the field conditions. The methods can also be used to classify rice genotypes according to their heat tolerance characteristics. Thus, different types of heat stress tolerance mechanisms can be identified, presenting opportunities for pyramiding different (mutant) sources of heat stress tolerance.
List of contents
General Introduction.- Screening Protocols for Heat Tolerance in Rice at the Seedling and Reproductive Stages.- Validation of Screening Protocols for Heat Tolerance in Rice.- Conclusion.- References.
About the author
Fatma Sarsu
Plant Breeding and Genetics Section
Joint FAO/IAEA Division
Vienna, Austria
Abdelbagi M.A. Ghanim
Plant Breeding and Genetics Laboratory
Joint FAO/IAEA Division
Vienna, Austria
Priyanka Das
School of Life Sciences
Jawaharlal Nehru University
New Delhi, India
Rajeev N Bahuguna
School of Life Sciences
Jawaharlal Nehru University
New Delhi, India
Paul Mbogo Kusolwa
Sokoine University of Agriculture
Morogoro, United Republic of Tanzania
Muhammed Ashraf
Nuclear Institute for Agriculture and Biology (NIAB)
Faisalabad, Pakistan
Sneh Lata Singla-Pareek Plant Molecular Biology Group
International Centre for Genetic Engineering and Biotechnology
New Delhi, India
Ashwani Pareek
School of Life Sciences
Jawaharlal Nehru University
New Delhi, India
Brian P. ForsterPlant Breeding and Genetics Laboratory Joint FAO/IAEA DivisionInternational Atomic Energy Agency Vienna, Austria Ivan Ingelbrecht
Plant Breeding and Genetics Laboratory
Joint FAO/IAEA Division International Atomic Energy Agency Vienna, Austria
