Read more
This second edition volume expands on the previous editions with updates on the commonly used techniques and analysis used by clinicians and scientists to study diabetes. The chapters in this book cover topics such as nutritional models of type 2 diabetes mellitus; the isolation and purification of rodent pancreatic islets of Langerhans; nitric oxide and redox state measurements in pancreatic beta cells; and primary adipocytes as a model for insulin sensitivity. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and practical, Type 2 Diabetes: Methods and Protocols, Second Edition is a valuable resource for all researchers who are interested in learning more about this important and evolving field.
List of contents
Sixty Years of Drug Discovery for Type 2 Diabetes: Where Are We Now?.- Practical Considerations for In Vivo Studies.- Nutritional Models of Type 2 Diabetes Mellitus.- Cheminformatics in the Identification of Drug Classes for the Treatment of Type 2 Diabetes.- Whole Exome Sequencing (WES) for Illumina HISeq using Solution-Based Capture.- Gene Expression Mining in Type 2 Diabetes Research.- Pathways Enrichment Analysis of Gene Expression Data in Type 2 Diabetes.- Diagnostic Genetic Testing for Monogenic Diabetes and Congenital Hyperinsulinemia.- The Isolation and Purification of Rodent Pancreatic Islets of Langerhans.- Characterization of Islet Leukocyte Populations in Human and Murine Islets by Flow Cytometry.- Analysis of Histone Modifications in Rodent Pancreatic Islets by Native Chromatin Immunoprecipitation.- Quantification of Pancreatic Islets: Using Image Analysis Tools.- The Measurement of Calcium Signaling In BetaCell Lines using Epifluorescence and Confocal Microscopy.- Nitric Oxide and Redox State Measurements in Pancreatic Beta Cells.- Primary Adipocytes as a Model for Insulin Sensitivity.- Assessing Islet Transplantation Outcome in Mice.- The Measurement of Insulin Secretion using Pancreas Perfusion in the Rodent.
Summary
This second edition volume expands on the previous editions with updates on the commonly used techniques and analysis used by clinicians and scientists to study diabetes. The chapters in this book cover topics such as nutritional models of type 2 diabetes mellitus; the isolation and purification of rodent pancreatic islets of Langerhans; nitric oxide and redox state measurements in pancreatic beta cells; and primary adipocytes as a model for insulin sensitivity. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and practical, T
ype 2 Diabetes: Methods and Protocols, Second Edition is a valuable resource for all researchers who are interested in learning more about this important and evolving field.
