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This volume looks at the importance of V. cholerae as an infection agent, and explores the latest techniques used to study it. The chapters in this book cover topics such as the biology of V. cholerae; laboratory maintenance and culturing; various animals models developed to explain pathogenesis and factors for environmental survival; and holistic approaches that include proteomics, metabolomics, and high-throughput screening. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and authoritative, Vibrio cholerae: Methods and Protocols is a valuable resource for both novice microbiologists who want to focus their studies on V. cholerae, and experienced researchers and teachers who want to improve their knowledge and skills in this growing field.
List of contents
Laboratory Culturing Techniques and Maintenance of Vibrio cholera.- Genotypic and Phenotypic Assays to Distinguish Vibrio cholera Biotype.- Preparation of Vibrio cholera Samples for RNA-seq Analysis.- Random Transposon Mutagenesis of Vibrio cholera.- Metabolomics of Vibrio cholera.- Natural Cotransformation and Multiplxed Genome Editing by Natural Transformation (MuGENT) of Vibrio cholera.- Vibrio cholera Chromatin Immunoprecipitation.- Fly Models of Vibrio cholera Infection and Colonization.- Danio rerio as a Native Host Model for Understanding Pathophysiology of Vibrio cholera.- Transposon Sequencing of Vibrio cholera in the Infant Rabbit Model of Cholera.- Isolation of Outer Membrane Vesicles Including their Quantitative and Qualitative Analyses.- Utilization of Vibrio cholera as a Model Organism to Screen natural Product Libraries for Identification of New Antibodies.- Infant Mouse Model of Vibrio cholera Infection and Colonization.- Methods for Assessments of Collagenolytic Activity of the Vibrio cholera Extracellular Proteases, Purification of Secreted Collagenase VchC, and Extraction of Type I Collagen from Fish Skin.- Proteomics of Vibrio cholerarea.
Summary
This volume looks at the importance of V. cholerae as an infection agent, and explores the latest techniques used to study it. The chapters in this book cover topics such as the biology of V. cholerae; laboratory maintenance and culturing; various animals models developed to explain pathogenesis and factors for environmental survival; and holistic approaches that include proteomics, metabolomics, and high-throughput screening. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and authoritative, Vibrio cholerae: Methods and Protocols is a valuable resource for both novice microbiologists who want to focus their studies on V. cholerae, and experienced researchers and teachers who want to improve their knowledge and skills in this growing field.
