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This detailed book explores methods to isolate, characterize, and investigate key lung innate immune cells. Beginning with an overview, the volume then continues with methods for creating in vitro and in vivo model systems to study inflammatory lung diseases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Lung Innate Immunity and Inflammation: Methods and Protocols aims to be a guidebook and will be of value and interest to researchers investigating innate immunity and inflammation in the lung as well as other organs and tissues.
List of contents
500 Million Alveoli from 30,000 Feet: A Brief Primer on Lung Anatomy.- Overview of Innate Lung Immunity and Inflammation.- Isolation and Characterization of Mononuclear Phagocytes in the Mouse Lung and Lymph Nodes.- Isolation and Characterization of Mouse Neutrophils.- Isolation and Characterization of Mouse Fibroblasts.- Isolation of Rat and Mouse Alveolar Type II Epithelial Cells.-Isolation and Characterization of Human Alveolar type II Cells.- Air-liquid Interface Culture of Human and Mouse Airway Epithelial Cells.- Isolation and Characterization of Human Lung Myeloid Cells.-Measurement of Protein Permeability and Fluid Transport of Human Alveolar Epithelial Type II Cells under Pathological Conditions.- Measuring Innate Immune Function in Mouse Mononuclear Phagocytes.- Measuring Neutrophil Bactericidal Activity.- Modulation of Myeloid Cell Function Using Conditional and Inducible Transgenic Approaches.- Modulation of Lung Epithelial Cell Function Using Conditional and Inducible Transgenic Approaches.- Computational Analysis of RNA-seq Data from Airway Epithelial Cells for Studying Lung Disease.- Application of Proteomics in Lung Research.- Application of Metabolomics in Lung Research.- Functional Genomics in Murine Macrophages.- Assessment of Ozone-induced Lung Injury in Mice.- Lung Histological Methods.- Intravital Microscopy in the Mouse Lung.- Mouse Models of Acute Lung Injury and ARDS.- Mouse Models of Asthma.- Animal Models of Pulmonary Fibrosis.- Mouse Models of COPD.- Mouse Models of COPD.- Delivery of Therapeutics to the Lung.
Summary
This detailed book explores methods to isolate, characterize, and investigate key lung innate immune cells. Beginning with an overview, the volume then continues with methods for creating in vitro and in vivo model systems to study inflammatory lung diseases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Lung Innate Immunity and Inflammation: Methods and Protocols aims to be a guidebook and will be of value and interest to researchers investigating innate immunity and inflammation in the lung as well as other organs and tissues.
