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This detailed book explores the most current techniques to study systems and epithelial cell culture. Beginning with an overview, the volume then continues to detail systems that seek to mimic the three-dimensional organization, epithelial cells from different organs, gastrointestinal system, thyroid, salivary gland, ovary, mammary gland, and olfactory epithelial tissue. Cell culture is a fundamental technique in both medical research and drug discovery and two-dimensional (2D) culture has been the preferred method, due to the ease with which cell monolayers can be induced to proliferate on planar surfaces. The book propose several functional assay useful to test cell activities. Further, The past decades have witnessed significant efforts toward the development of three-dimensional (3D) cell cultures. Today, 3D cell cultures are emerging not only as a new tool in early drug discovery, but also as potential therapeutics to treat disease. Written forthe highly successful Methods in Molecular Biology series, chapters include the kind of detail and key implementation advice that leads to excellent results in the lab.
List of contents
Normal Human Thyrocytes in Culture.- Isolation and Culture of Juvenile Pig Thyroid Follicular Epithelia.- Reassembly of Functional Human Salivary Stem/Progenitor Cells in 3D Culture.- Culture and Differentiation of Lung Bronchiolar Epithelial Cells In Vitro.- Differentiation of gastrointestinal cell lines by culture in semi-wet interface.- Three-dimensional cell culture model utilization in renal carcinoma cancer stem cell research.- Amniotic epithelial cell culture. Corresponding author.- Bovine granulosa cell culture.- Bioencapsulation of oocytes and granulosa cells.- Ovine granulosa cells isolation and culture to improve oocyte quality.- 3D model replicating the intestinal function to evaluate drug permeability.- Isolation of Human Gastric Epithelial Cells from Gastric Surgical Tissue and Gastric Biopsies for Primary Culture.- Long-term culture of intestinal organoids.- Bovine mammary organoids: a model to study epithelial mammary cells.- Establishment of Human- and Mouse-Derived Gastric Primary Epithelial Cell Monolayers from organoids.- Mouse-Derived Gastric Organoid and Immune Cell Co-Culture for the Study of the Tumor Microenvironment.- Murine and human mammary cancer cell lines: functional tests.- In vitro porcine colon culture.- Primary cultures of olfactory neurons from the avian olfactory epithelium.
About the author
Mario Baratta is Full professor in Veterinary Physiology at the University of Turin (IT). He got the master degree in Veterinary Medicine at the University of Parma (IT) and the PhD in Neuroendocrinology of Animal Farm at the University of Bologna (IT). Today he is the Coordinator of the Graduate School in Veterinary Science for Animal Health and Food Safety, University of Turin. His main interests are the biology of adult stem cells in different tissues and he has developed specific knowledge in functional assessment systems of epithelial cells and cell differentiation in mammary gland and in muscle in animal science.
Summary
This detailed book explores the most current techniques to study systems and epithelial cell culture. Beginning with an overview, the volume then continues to detail systems that seek to mimic the three-dimensional organization, epithelial cells from different organs, gastrointestinal system, thyroid, salivary gland, ovary, mammary gland, and olfactory epithelial tissue. Cell culture is a fundamental technique in both medical research and drug discovery and two-dimensional (2D) culture has been the preferred method, due to the ease with which cell monolayers can be induced to proliferate on planar surfaces. The book propose several functional assay useful to test cell activities. Further, The past decades have witnessed significant efforts toward the development of three-dimensional (3D) cell cultures. Today, 3D cell cultures are emerging not only as a new tool in early drug discovery, but also as potential therapeutics to treat disease. Written forthe highly successful Methods in Molecular Biology series, chapters include the kind of detail and key implementation advice that leads to excellent results in the lab.
