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This book details methods on various aspects of the very final stages of mouse oocyte development. Chapters guide the reader through in vitro growth of follicles, production of a fully-grown competent mouse oocyte, meiosis resumption, analysis of spindle assembly and chromosome segregation, analysis of the oocyte and early embryo transcriptome. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Mouse Oocyte Development: Methods and Protocols aims to help scientists with the set up both live and fix experiments required to study the process of oocyte meiotic maturation.
List of contents
Growth In Vitro of Granulosa Cell-oocyte Complexes of the Mouse.- In vitro Culture of Mouse Oocytes for Meiotic Maturation.- In vitro Maturation and Culture of Human Oocytes.- Pronuclear Transfer in Human Oocytes.- In vitro Fertilization of Mouse Oocyte.- Profiling Maternal mRNA Translation during Oocyte Development.- Transcriptome Profiling of Single Mouse Oocytes.- Immunofluorescence Technique to Detect Subcellular Structures Critical to Oocyte Maturation.- Immunofluorescence Staining of K-fibres in Mouse Oocytes Using Cold-fixation.- Triple-colour Live Imaging of Mouse Oocytes.- Detection of Separase Activity Using a Cleavage Sensor in Live Mouse Oocytes.- Manipulating Cohesin Levels in Live Mouse Oocytes.- Optogenetic Manipulation of Mouse Oocytes.- Monitoring Microtubule Dynamics in the Mouse Egg using PhotoActivatableGFP-tubulin.- Photoactivation of Actin in Mouse Oocyte.- Laser Ablation of Microtubule-chromosome Attachment in Mouse Oocytes.- Micropipette Aspiration of Oocytes to Assess Cortical Tension.
Summary
This book details methods on various aspects of the very final stages of mouse oocyte development. Chapters guide the reader through in vitro growth of follicles, production of a fully-grown competent mouse oocyte, meiosis resumption, analysis of spindle assembly and chromosome segregation, analysis of the oocyte and early embryo transcriptome. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Mouse Oocyte Development: Methods and Protocols aims to help scientists with the set up both live and fix experiments required to study the process of oocyte meiotic maturation.
Additional text
“Each chapter presents not only detailed and reproducible methods (troubleshooting included) but also nice images and instructive figures providing non-verbal information necessary to better understand how to deal with this ‘fantastic little laboratory of molecular biology’ that is the oocyte.” (Manuela Monti, European Journal of Histochemistry, Vol. 62 (04), October, 2018)
Report
"Each chapter presents not only detailed and reproducible methods (troubleshooting included) but also nice images and instructive figures providing non-verbal information necessary to better understand how to deal with this 'fantastic little laboratory of molecular biology' that is the oocyte." (Manuela Monti, European Journal of Histochemistry, Vol. 62 (04), October, 2018)
