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This volume provides a collection of state-of-the-art protocols on plant vacuolar trafficking. Chapters guide the reader through design and preparation of fusion proteins, expression vectors, and detailed analysis of different microscopy techniques. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Plant Vacuolar Trafficking: Methods and Protocols aims to be a highly valuable resource for researchers interested in learning more about this field
List of contents
Green-fluorescent Seed, to Evaluate Vacuolar Trafficking in Arabidopsis Seeds.- Detection of CRISPR/Cas9 Induced Genomic Fragment Deletions in Barley and Generation of Homozygous Edited Lines via Embryogenic Pollen Culture.- Vacuolar Sorting Determinants - Isolation and Study.- Vacuole-targeted Proteins: Ins and Outs of Subcellular Localization Studies.- Plant Cell Vacuoles: Staining and Fluorescent Probes.- Vacuolar Targeting and Characterization of Recombinant Antibodies.- Isolation of Vacuoles from the Leaves of the Medicinal Plant Catharanthus roseus.- Flow Cytometry and Fluorescence Microscopy as Tools for Structural and Functional Analysis of Vacuoles Isolated from Yeast and Plant Cells.- Live cell Imaging and Confocal Microscopy.- Imaging Vacuolar Anthocyanins with Fluorescence Lifetime Microscopy (FLIM).- The Use of Drugs in the Study of Vacuole Morphology and Trafficking to the Vacuole in Arabidopsis thaliana.- Use of Brefeldin A and Wortmannin to Dissect Post-Golgi OrganellesRelated to Vacuolar Transport in Arabidopsis thaliana.- Use Endosidin2 to Study Plant Exocytosis and Vacuolar Trafficking.- Visualization of RMRs (Receptor Membrane RING-H2) Dimerization in Nicotiana Benthamiana Leaves using a Bimolecular Fluorescence Complementation (BiFC) Assay.- Vacuole Dynamics in Rice Cells Invaded by the Blast Fungus Magnaporthe Oryzae.- Characterization Of Plant Glycoproteins: Analysis of Plant Glycopeptide Mass Spectrometry Data with plantGlycoMS, a Package in the R Statistical Computing Environment.
Summary
This volume provides a collection of state-of-the-art protocols on plant vacuolar trafficking. Chapters guide the reader through design and preparation of fusion proteins, expression vectors, and detailed analysis of different microscopy techniques. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Plant Vacuolar Trafficking: Methods and Protocols aims to be a highly valuable resource for researchers interested in learning more about this field
