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This volume explores disease diagnosis and therapy in developing theranostics. The chapters in this book are divided into four parts and cover various techniques used in studying bioengineering, molecular diagnostics, in vivo imaging, and imaging-guided therapy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Theranostics: Methods and Protocols is a valuable tool for novice and expert clinicians, researchers, and students working in this multidisciplinary field.
List of contents
Cell Recovery of Hydrogel-Encapsulated Cells for Molecular Analysis.- Bioengineered Microtissue Models of the Human Bone Metastatic Microenvironment: A Novel In Vitro Theranostics Platform for Cancer Research.- Real-Time and 3D Quantification of Cancer Cell Dynamics: Exploiting a Bioengineered Human Bone Metastatic Microtissue.- Exomes Extraction and Identification.- Profiling MicroRNA Markers in Plasma: Looking into Better Approaches and Recommendations.- Isolation and Quantification of MicroRNAs from Human Saliva.- The Isolation and Analysis of Circulating Tumor Cells.- The Isolation and Characterization of Circulating Tumor Cells from Head and Neck Cancer Patient Blood Samples using Spiral Microfluidic Technology.- Pharmacogenetics: Role of Genetic Variants - SNPs.- EGFR Mutation Analysis in Non-Small Cell Lung Carcinoma from Tissue Samples Using the Fully Automated Idylla(TM) qPCR System.- Subcellular Localization of MicroRNAs by MicroRNA In Situ Hybridization (miR-ISH).- Digital Holographic Imaging as a Method for Quantitative, Live Cell Imaging of Drug Response to Novel Targeted Cancer Therapies.- Luminescent Porous Silicon Nanoparticles for Continuous Wave and Time-Gated Photoluminescence Imaging.- Nucleic Acid Aptamers as Emerging Tools for Diagnostics and Theranostics.- Aptamer Selection for Detecting Molecular Target using Cell-SELEX (Systematic Evolution of Ligands by Exponential Enrichment) Technology.- Fluorescence In Situ Hybridization and Rehybridization Using Bacterial Artificial Chromosome Probes.- Upconversion Nanoparticle-Base Strategy for Blood-Brain Barrier across for Central Nervous Disease Therapy.- Genetically Engineered Plasma Membrane Nanovesicles for Cancer-Targeted Nanotheranostics.
Summary
This volume explores disease diagnosis and therapy in developing theranostics. The chapters in this book are divided into four parts and cover various techniques used in studying bioengineering, molecular diagnostics, in vivo imaging, and imaging-guided therapy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and comprehensive, Theranostics: Methods and Protocols is a valuable tool for novice and expert clinicians, researchers, and students working in this multidisciplinary field.
