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This detailed book provides a compilation of laboratory techniques and tests to assess the risks to embryo-fetal development from drug exposure during early developmental stages. After an introduction to teratogenicity testing, the contents explore both in vitro and in vivo techniques in the study of the teratogenic and fetotoxic effects of drugs, as well as numerous animal-based teratology methods. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and comprehensive, Teratogenicity Testing: Methods and Protocols describes methods that integrate a battery of tests that can be performed in cells, organs, tissues, and animal models for evaluating toxicity and/or the safety of compounds in early developmental stages with the goal of estimating, preventing, or minimizing the teratogenic potential of drugs.
List of contents
An Overview of Teratology.- Teratology Study Guidelines: An Overview.- Biological Concerns on the Selection of Animal Models for Teratogenic Testing.- The Validated Embryonic Stem Cell Test with Murine Embryonic Stem Cells.- Human Pluripotent Stem Cells to Assess Developmental Toxicity in the Osteogenic Lineage.- Chick Embryonic Cardiomyocytes Micromass System for Assessing Developmental Cardiotoxicity of Drugs.- Flow Cytometry to Evaluate Potential Developmental Toxicants in the Embryonic Stem Cell.- Morphology-Based Whole Embryo Culture for Developmental Toxicity of Drugs.- Western Blot Methodologies for Analysis of In Vitro Protein Expression Induced by Teratogenic Agents.- Manipulation of MicroRNAs in Cultured Mouse Embryos: Applications for Developmental Toxicology.- Insights into the Phenotypic and Behavioral Effects of Teratogenic Drugs in Caenorhabditis elegans.- Effect of Teratogens on Development of Drosophila melanogaster.- Cellular Responses in Drosophila melanogaster Following Teratogen Exposure.- Behavioral Teratogenesis in Drosophila melanogaster.- Evaluation of the Teratogenicity of Pharmaceuticals Using FETAX.- Histological Observation of Teratogenic Phenotypes Induced in Frog Embryo Assays.- Visualization of Gene Expression Patterns by In Situ Hybridization on Early Stages of Development of Xenopus laevis.- Analysis of Lethality and Malformations During Zebrafish (Danio rerio) Development.- General Whole-Mount Immunohistochemistry of Zebrafish (Danio rerio) Embryos and Larvae Protocol.- Geometric Morphometrics as a Tool to Evaluate Teratogenic Effects in Zebrafish (Danio rerio).- Live Metabolic Profile Analysis of Zebrafish Embryos Using a Seahorse XF 24 Extracellular Flux Analyzer.- Behavioral Profiling of Zebrafish (Danio rerio) Larvae Following Teratogen Exposure.- Omics in Zebrafish Teratogenesis.- Proteomic Analysis of Zebrafish (Danio rerio) after Chemical Exposure.- Immunohistochemical Assessment as a Tool for Investigating Developmental Toxicity in Zebrafish (Danio rerio).- Oxidative Stress Assessment in Zebrafish Larvae.- Hemodynamic Studies for Analyzing the Teratogenic Effects of Drugs in the Zebrafish Embryo.- Western Blot Analysis and Immunostaining for Prediction of Embryotoxicity in Mus musculus.- Histological and Histochemical Profile for Teratological Assessment in Mus musculus.- In Vivo Analysis of Apoptosis in Embryonic Hippocampus.- Measurement of Mitochondrial Toxicity Parameters in Embryonic Hippocampus.- Animal Tests for Evaluation of Cognitive Impairment in Neonatal Mouse.- Methodology of Genotoxic and Teratogenic Studies in Rats.- Whole Mount In Situ Hybridization and Morphometric Analysis in Rabbit Embryos.- Glossary of Key Terms for Teratology.
Summary
This detailed book provides a compilation of laboratory techniques and tests to assess the risks to embryo-fetal development from drug exposure during early developmental stages. After an introduction to teratogenicity testing, the contents explore both in vitro and in vivo techniques in the study of the teratogenic and fetotoxic effects of drugs, as well as numerous animal-based teratology methods. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and comprehensive, Teratogenicity Testing: Methods and Protocols describes methods that integrate a battery of tests that can be performed in cells, organs, tissues, and animal models for evaluating toxicity and/or the safety of compounds in early developmental stages with the goal of estimating, preventing, or minimizing the teratogenic potential of drugs.
