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This book explores the vital importance of T-cell differentiation in areas as wide-ranging as pathological analysis, drug development, and cell therapy of human T-cells. Focusing on human embryonic stem cells and human induced pluripotent stem cells, the chapters explore a variety of in vitro T-cell differentiation protocols as well as useful techniques to develop and evaluate cellular medicines. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, In Vitro Differentiation of T-Cells: Methods and Protocols serves as an ideal guide for researchers seeking to differentiate T-cells from pluripotent stem cells in order to achieve any number of significant goals.
List of contents
Flow Cytometry Analysis to Identify Human CD8+ T-Cells.- Flow Cytometry Analysis to Identify Human CD4+ T-Cell Subsets.- Gene Modification and Immunological Analyses for the Development of Immunotherapy Utilizing T-Cells Redirected with Antigen-Specific Receptors.- In Vitro Conversion of Activated T-Cells into Stem Cell Memory-Like T-Cells.- Human iPSC Generation from Antigen-Specific T-Cells.- In Vitro Differentiation of T-Cells: From Human Embryonic Stem Cells and Induced Pluripotent Stem Cells.- Redifferentiation of Adaptive Naïve-Like CTL from T-Cell-Derived iPSC.- In Vitro Differentiation of T-Cell: From Human iPS Cells in Feeder Free Condition.- Differentiating CD8ab T-Cells from TCR Transduced iPSCs for Cancer Immunotherapy.- In Vitro Differentiation of T-Cell: From CAR-Modified T-iPSC.- In Vitro Differentiation of T-Cells: From Non-Human Primate Induced Pluripotent Stem Cells.- An Improved Method to Produce Clinical Scale Natural Killer Cells from Human Pluripotent Stem Cells.- In Vitro Detection of Cellular Adjuvant Properties of Human Invariant Natural Killer T-Cells.- In Vitro Differentiation of T-Cells from Murine Pluripotent Stem Cells.- Clonogenic Culture of Mouse Thymic Epithelial Cells.- Single-Cell Transcriptome Analysis of T-Cells.- Structural Modeling of Lymphocyte Receptors and Their Antigens.- Assessing T Lymphocyte Aging Using Telomere Length and Telomerase Activity Measurements in Low Cell Numbers.- Generation of Hematopoietic Stem and Progenitor Cells from Human Pluripotent Stem Cells.- Using the Inducible Caspase9 Suicide-Safeguard System with iPSC and Bioluminescent Tracking.
Summary
This book explores the vital importance of T-cell differentiation in areas as wide-ranging as pathological analysis, drug development, and cell therapy of human T-cells. Focusing on human embryonic stem cells and human induced pluripotent stem cells, the chapters explore a variety of in vitro T-cell differentiation protocols as well as useful techniques to develop and evaluate cellular medicines. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, In Vitro Differentiation of T-Cells: Methods and Protocols serves as an ideal guide for researchers seeking to differentiate T-cells from pluripotent stem cells in order to achieve any number of significant goals.
