Read more
This volume covers the structures, properties, and functions of G-quadruplexes in a wide range of biological disciplines, including therapeutic intervention and biomaterial application. The chapters in this book explore a wide range of vital and new experimental techniques used in the study of G-quadruplexes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Practical and cutting-edge, G-Quadruplex DNA: Methods and Protocols is a valuable resource for both novice and experienced researchers who work in biophysics, structural biology, computational biology, biochemistry, and molecular and cell biology, and who want to learn more about the potential roles and effects of G-quadruplex in these fields.
List of contents
G-Quadruplex DNA and RNA.- CD Study of the G-Quadruplex Conformation.- Revealing the Energetics of Ligand-Quadruplex Interactions using Isothermal Titration Calorimetry.- Biosensor-Surface Plasmon Resonance: Label Free Method for Investigation of Small Molecule-Quadruplex Nucleic Acid Interactions.- Putting a New Spin of G-Quadruplex Structure and Binding by Analytical Ultracentrifugation.- Mass Spectroscopic Study of G-Quadruplex.- G-Quadruplex Stability from DSC Measurements.- X-Ray Crystallographic Studies of G-Quadruplex Structures.- NMR Studies of G-Quadruplex Structures and G-Quadruplex-Interactive Compounds.- Using Molecular Dynamics Free Energy Simulation to Compute Binding Affinities of DNA G-Quadruplex Ligands.- Electrophoretic Mobility Shift Assay and Dimethyl Sulfate Footprinting for Characterization of G-Quadruplexes and G-Quadruplex-Protein Complexes.- A DNA Polymerase Stop Assay for Characterization of G-Quadruplex Formation and Identification of G-Quadruplex-Interactive Compounds.- Chromatin Immunoprecipitation Assay to Analyze the Effect of G-Quadruplex Interactive Agents on the Binding of RNA Polymerase II and Transcription Factors to a Target Promoter Region.- Characterization of Co-Transcriptional Formation of G-Quadruplexes in Double-Stranded DNA.- Quantitative Analysis of Stall of Replicating DNA Polymerase by G-Quadruplex Formation.- Single-Molecule Investigations of G-Quadruplex.- Direct Observation of the Formation and Dissociation of Double-Stranded DNA Containing G-Quadruplex/i-motif Sequences in the DNA Origami Frame using High-Speed AFM.- G-Quadruplex and Protein Binding by Single-Molecule FRET Microscopy.- High-Throughput Screening of G-Quadruplex Ligands by FRET Assay.- Targeting G-Quadruplexes with PNA Oligomers.- Primer-Modified G-Quadruplex-Au Nanoparticles for Colorimetric Assay of Human Telomerase Activity and Initial Screening of Telomerase Inhibitors.- Heme-G-Quadruplex DNAzymes: Conditions for Maximizing their Peroxidase Activity.- In Vivo Chemical Probing for G-Quadruplex Formation.- G-Quadruplex Visualization In Cells via Antibody and Fluorescence Probe.- In Cell NMR Spectroscopy: Investigation of G-Quadruplex Structures inside Living Xenopus laevis Oocytes.- 19F NMR Spectroscopy for the Analysis of DNA G-Quadruplex Structures using 19F-labeled Nucleobase.
Summary
This volume covers the structures, properties, and functions of G-quadruplexes in a wide range of biological disciplines, including therapeutic intervention and biomaterial application. The chapters in this book explore a wide range of vital and new experimental techniques used in the study of G-quadruplexes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Practical and cutting-edge, G-Quadruplex DNA: Methods and Protocols is a valuable resource for both novice and experienced researchers who work in biophysics, structural biology, computational biology, biochemistry, and molecular and cell biology, and who want to learn more about the potential roles and effects of G-quadruplex in these fields.
